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Egg yolk immunoglobulin (IgY) targeting SARS-CoV-2 S1 as potential virus entry blocker.
Bao, Lirong; Zhang, Cheng; Lyu, Jinglu; Yi, Ping; Shen, Xin; Tang, Boyu; Zhao, Hang; Ren, Biao; Kuang, Yu; Zhou, Linlin; Li, Yan.
  • Bao L; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Zhang C; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Lyu J; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Yi P; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Shen X; The Clinical Laboratory of West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  • Tang B; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Zhao H; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Ren B; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Kuang Y; State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
  • Zhou L; Department of Microbiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.
  • Li Y; Department of Microbiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.
J Appl Microbiol ; 132(3): 2421-2430, 2022 Mar.
Article in English | MEDLINE | ID: covidwho-1488216
ABSTRACT

AIMS:

COVID-19 pandemic caused by SARS-CoV-2 has become a public health crisis worldwide. In this study, we aimed at demonstrating the neutralizing potential of the IgY produced after immunizing chicken with a recombinant SARS-CoV-2 spike protein S1 subunit. METHODS AND

RESULTS:

E. coli BL21 carrying plasmid pET28a-S1 was induced with IPTG for the expression of SARS-CoV-2 S1 protein. The recombinant His-tagged S1 was purified and verified by SDS-PAGE, Western blot and biolayer interferometry (BLI) assay. Then S1 protein emulsified with Freund's adjuvant was used to immunize layer chickens. Specific IgY against S1 (S1-IgY) produced from egg yolks of these chickens exhibited a high titer (125,600) and a strong binding affinity to S1 (KD  = 318 nmol L-1 ). The neutralizing ability of S1-IgY was quantified by a SARS-CoV-2 pseudotyped virus-based neutralization assay with an IC50  value of 0.99 mg ml-1 . In addition, S1-IgY exhibited a strong ability in blocking the binding of SARS-CoV-2 S1 to hACE2, and it could partially compete with hACE2 for the binding sites on S1 by BLI assays.

CONCLUSIONS:

We demonstrated here that after immunization of chickens with our recombinant S1 protein, IgY neutralizing antibodies were generated against the SARS-CoV-2 spike protein S1 subunit; therefore, showing the potential use of IgY to block the entry of this virus. SIGNIFICANCE AND IMPACT OF THE STUDY IgY targeting S1 subunit of SARS-CoV-2 could be a promising candidate for pre- and post-exposure prophylaxis or treatment of COVID-19. Administration of IgY-based oral preparation, oral or nasal spray may have profound implications for blocking SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulins / Virus Internalization / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / SARS-CoV-2 Type of study: Experimental Studies Limits: Animals / Humans Language: English Journal: J Appl Microbiol Journal subject: Microbiology Year: 2022 Document Type: Article Affiliation country: Jam.15340

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulins / Virus Internalization / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / SARS-CoV-2 Type of study: Experimental Studies Limits: Animals / Humans Language: English Journal: J Appl Microbiol Journal subject: Microbiology Year: 2022 Document Type: Article Affiliation country: Jam.15340