Biphasic effect of mechanical stress on lymphocyte activation.
J Cell Physiol
; 237(2): 1521-1531, 2022 02.
Article
in English
| MEDLINE | ID: covidwho-1490820
ABSTRACT
Mechanical forces can modulate the immune response, mostly described as promoting the activation of immune cells, but the role and mechanism of pathological levels of mechanical stress in lymphocyte activation have not been focused on before. By an ex vivo experimental approach, we observed that mechanical stressing of murine spleen lymphocytes with 50 mmHg for 3 h induced the nuclear localization of NFAT1, increased C-Jun, and increased the expression of early activation marker CD69 in resting CD8+ cells. Interestingly, 50 mmHg mechanical stressing induced the nuclear localization of NFAT1; but conversely decreased C-Jun and inhibited the expression of CD69 in lymphocytes under lipopolysaccharide or phorbol 12-myristate 13-acetate/ionomycin stimulation. Additionally, we observed similar changes trends when comparing RNA-seq data of hypertensive and normotensive COVID-19 patients. Our results indicate a biphasic effect of mechanical stress on lymphocyte activation, which provides insight into the variety of immune responses in pathologies involving elevated mechanical stress.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Stress, Mechanical
/
Lymphocyte Activation
Type of study:
Experimental Studies
/
Prognostic study
Topics:
Long Covid
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
J Cell Physiol
Year:
2022
Document Type:
Article
Affiliation country:
Jcp.30623
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