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SARS-CoV-2 can infect and propagate in human placenta explants.
Fahmi, Amal; Brügger, Melanie; Démoulins, Thomas; Zumkehr, Beatrice; Oliveira Esteves, Blandina I; Bracher, Lisamaria; Wotzkow, Carlos; Blank, Fabian; Thiel, Volker; Baud, David; Alves, Marco P.
  • Fahmi A; Institute of Virology and Immunology, Bern, Switzerland.
  • Brügger M; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Démoulins T; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
  • Zumkehr B; Institute of Virology and Immunology, Bern, Switzerland.
  • Oliveira Esteves BI; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Bracher L; Institute of Virology and Immunology, Bern, Switzerland.
  • Wotzkow C; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Blank F; Institute of Virology and Immunology, Bern, Switzerland.
  • Thiel V; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
  • Baud D; Institute of Virology and Immunology, Bern, Switzerland.
  • Alves MP; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Cell Rep Med ; 2(12): 100456, 2021 12 21.
Article in English | MEDLINE | ID: covidwho-1500334
ABSTRACT
The ongoing SARS-CoV-2 pandemic continues to lead to high morbidity and mortality. During pregnancy, severe maternal and neonatal outcomes and placental pathological changes have been described. We evaluate SARS-CoV-2 infection at the maternal-fetal interface using precision-cut slices (PCSs) of human placenta. Remarkably, exposure of placenta PCSs to SARS-CoV-2 leads to a full replication cycle with infectious virus release. Moreover, the susceptibility of placental tissue to SARS-CoV-2 replication relates to the expression levels of ACE2. Viral proteins and/or viral RNA are detected in syncytiotrophoblasts, cytotrophoblasts, villous stroma, and possibly Hofbauer cells. While SARS-CoV-2 infection of placenta PCSs does not cause a detectable cytotoxicity or a pro-inflammatory cytokine response, an upregulation of one order of magnitude of interferon type III transcripts is measured. In conclusion, our data demonstrate the capacity of SARS-CoV-2 to infect and propagate in human placenta and constitute a basis for further investigation of SARS-CoV-2 biology at the maternal-fetal interface.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Placenta / SARS-CoV-2 Type of study: Experimental Studies Limits: Female / Humans / Pregnancy Language: English Journal: Cell Rep Med Year: 2021 Document Type: Article Affiliation country: J.xcrm.2021.100456

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Placenta / SARS-CoV-2 Type of study: Experimental Studies Limits: Female / Humans / Pregnancy Language: English Journal: Cell Rep Med Year: 2021 Document Type: Article Affiliation country: J.xcrm.2021.100456