Your browser doesn't support javascript.
Molecular diagnosis of SARS-CoV-2 in seminal fluid.
Paoli, D; Pallotti, F; Nigro, G; Mazzuti, L; Hirsch, M N; Valli, M B; Colangelo, S; Mastroianni, C M; Antonelli, G; Lenzi, A; Turriziani, O; Lombardo, F.
  • Paoli D; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy. donatella.paoli@uniroma1.it.
  • Pallotti F; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Nigro G; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Mazzuti L; Laboratory of Virology, Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
  • Hirsch MN; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Valli MB; National Institute for Infectious Diseases, INMI (Istituto Nazionale Per Le Malattie Infettive), "Lazzaro Spallanzani" IRCCS, Rome, Italy.
  • Colangelo S; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Mastroianni CM; Department of Public Health and Infectious Diseases, "Sapienza" University of Rome, Rome, Italy.
  • Antonelli G; Laboratory of Virology, Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
  • Lenzi A; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Turriziani O; Laboratory of Virology, Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
  • Lombardo F; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
J Endocrinol Invest ; 44(12): 2675-2684, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1504521
Semantic information from SemMedBD (by NLM)
1. Reverse Transcriptase Polymerase Chain Reaction METHOD_OF Detection
Subject
Reverse Transcriptase Polymerase Chain Reaction
Predicate
METHOD_OF
Object
Detection
2. Cultured Cell Line LOCATION_OF Virus
Subject
Cultured Cell Line
Predicate
LOCATION_OF
Object
Virus
3. Assay USES PRM1 gene|PRM1
Subject
Assay
Predicate
USES
Object
PRM1 gene|PRM1
4. Assay USES PRM2 gene|PRM2
Subject
Assay
Predicate
USES
Object
PRM2 gene|PRM2
5. Reverse Transcriptase Polymerase Chain Reaction METHOD_OF Detection
Subject
Reverse Transcriptase Polymerase Chain Reaction
Predicate
METHOD_OF
Object
Detection
6. Cultured Cell Line LOCATION_OF Virus
Subject
Cultured Cell Line
Predicate
LOCATION_OF
Object
Virus
7. Assay USES PRM1 gene|PRM1
Subject
Assay
Predicate
USES
Object
PRM1 gene|PRM1
8. Assay USES PRM2 gene|PRM2
Subject
Assay
Predicate
USES
Object
PRM2 gene|PRM2
ABSTRACT

PURPOSE:

Due to relevant repercussions on reproductive medicine, we aimed to evaluate feasibility of RT-PCR as a detection method of SARS-CoV-2 RNA in seminal fluid.

METHODS:

A qualitative determination of the RT-PCR assays in semen was performed through different approaches (1) efficiency of RNA extraction from sperm and seminal plasma was determined using PRM1 and PRM2 mRNA and a heterologous system as control; (2) samples obtained by diluting viral preparation from a SARS-CoV-2 panel (virus cultured in Vero E6 cell lines) were tested; (3) viral presence in different fractions of seminal fluid (whole sample, seminal plasma and post-centrifugation pellet) was evaluated. Semen samples from mild and recovered COVID-19 subjects were collected by patients referring to the Infectious Disease Department of the Policlinico Umberto I Hospital - "Sapienza" University of Rome. Control subjects were recruited at the Laboratory of Seminology-Sperm Bank "Loredana Gandini'' of the same hospital.

RESULTS:

The control panel using viral preparations diluted in saline and seminal fluid showed the capability to detect viral RNA presence with Ct values depending on the initial viral concentration. All tested semen samples were negative for SARS-CoV-2, regardless of the nasopharyngeal swab result or seminal fluid fraction.

CONCLUSION:

These preliminary data show that RT-PCR for SARS-CoV-2 RNA testing appears to be a feasible method for the molecular diagnosis of SARS-CoV-2 in seminal fluid, supported by results of the control panel. The ability to detect SARS-CoV-2 in semen is extremely important for reproductive medicine, especially in assisted reproductive technology and sperm cryopreservation.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Semen / Pathology, Molecular / COVID-19 Subject: Semen / Pathology, Molecular / COVID-19 Type of study: Diagnostic study / Qualitative research Language: English Journal: J Endocrinol Invest Clinical aspect: Diagnosis Year: 2021

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Semen / Pathology, Molecular / COVID-19 Subject: Semen / Pathology, Molecular / COVID-19 Type of study: Diagnostic study / Qualitative research Language: English Journal: J Endocrinol Invest Clinical aspect: Diagnosis Year: 2021
...