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Molecular diagnosis of SARS-CoV-2 in seminal fluid.
Paoli, D; Pallotti, F; Nigro, G; Mazzuti, L; Hirsch, M N; Valli, M B; Colangelo, S; Mastroianni, C M; Antonelli, G; Lenzi, A; Turriziani, O; Lombardo, F.
  • Paoli D; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy. donatella.paoli@uniroma1.it.
  • Pallotti F; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Nigro G; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Mazzuti L; Laboratory of Virology, Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
  • Hirsch MN; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Valli MB; National Institute for Infectious Diseases, INMI (Istituto Nazionale Per Le Malattie Infettive), "Lazzaro Spallanzani" IRCCS, Rome, Italy.
  • Colangelo S; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Mastroianni CM; Department of Public Health and Infectious Diseases, "Sapienza" University of Rome, Rome, Italy.
  • Antonelli G; Laboratory of Virology, Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
  • Lenzi A; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
  • Turriziani O; Laboratory of Virology, Department of Molecular Medicine, "Sapienza" University of Rome, Rome, Italy.
  • Lombardo F; Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.
J Endocrinol Invest ; 44(12): 2675-2684, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1504521
ABSTRACT

PURPOSE:

Due to relevant repercussions on reproductive medicine, we aimed to evaluate feasibility of RT-PCR as a detection method of SARS-CoV-2 RNA in seminal fluid.

METHODS:

A qualitative determination of the RT-PCR assays in semen was performed through different approaches (1) efficiency of RNA extraction from sperm and seminal plasma was determined using PRM1 and PRM2 mRNA and a heterologous system as control; (2) samples obtained by diluting viral preparation from a SARS-CoV-2 panel (virus cultured in Vero E6 cell lines) were tested; (3) viral presence in different fractions of seminal fluid (whole sample, seminal plasma and post-centrifugation pellet) was evaluated. Semen samples from mild and recovered COVID-19 subjects were collected by patients referring to the Infectious Disease Department of the Policlinico Umberto I Hospital - "Sapienza" University of Rome. Control subjects were recruited at the Laboratory of Seminology-Sperm Bank "Loredana Gandini'' of the same hospital.

RESULTS:

The control panel using viral preparations diluted in saline and seminal fluid showed the capability to detect viral RNA presence with Ct values depending on the initial viral concentration. All tested semen samples were negative for SARS-CoV-2, regardless of the nasopharyngeal swab result or seminal fluid fraction.

CONCLUSION:

These preliminary data show that RT-PCR for SARS-CoV-2 RNA testing appears to be a feasible method for the molecular diagnosis of SARS-CoV-2 in seminal fluid, supported by results of the control panel. The ability to detect SARS-CoV-2 in semen is extremely important for reproductive medicine, especially in assisted reproductive technology and sperm cryopreservation.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Semen / Pathology, Molecular / COVID-19 Type of study: Diagnostic study / Experimental Studies / Qualitative research Limits: Adult / Animals / Humans / Male Language: English Journal: J Endocrinol Invest Year: 2021 Document Type: Article Affiliation country: S40618-021-01580-x

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Semen / Pathology, Molecular / COVID-19 Type of study: Diagnostic study / Experimental Studies / Qualitative research Limits: Adult / Animals / Humans / Male Language: English Journal: J Endocrinol Invest Year: 2021 Document Type: Article Affiliation country: S40618-021-01580-x