Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.
Virus Res
; 307: 198618, 2022 01 02.
Article
in English
| MEDLINE | ID: covidwho-1504602
ABSTRACT
The second wave of COVID-19 caused by severe acute respiratory syndrome virus (SARS-CoV-2) is rapidly spreading over the world. Mechanisms behind the flee from current antivirals are still unclear due to the continuous occurrence of SARS-CoV-2 genetic variants. Brazil is the world's second-most COVID-19 affected country. In the present study, we identified the genomic and proteomic variants of Brazilian SARS-CoV-2 isolates. We identified 16 different genotypic variants were found among the 27 isolates. The genotypes of three isolates such as Bra/1236/2021 (G15), Bra/MASP2C844R2/2020 (G11), and Bra/RJ-DCVN5/2020 (G9) have a unique mutant in NSP4 (S184N), 2'O-Mutase (R216N), membrane protein (A2V) and Envelope protein (V5A). A mutation in RdRp of SARS-CoV-2, particularly the change of Pro-to Leu-at 323 resulted in the stabilization of the structure in BRA/CD1739-P4/2020. NSP4, NSP5 protein mutants are more virulent in genotype 15 and 16. A fast protein folding rate changes the structural stability and leads to escape for current antivirals. Thus, our findings help researchers to develop the best potent antivirals based on the new mutant of Brazilian isolates.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viral Nonstructural Proteins
/
Protein Folding
/
Coronavirus 3C Proteases
/
SARS-CoV-2
Topics:
Variants
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Virus Res
Journal subject:
Virology
Year:
2022
Document Type:
Article
Affiliation country:
J.virusres.2021.198618
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