Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light.
Vox Sang
; 115(3): 146-151, 2020 Apr.
Article
in English
| MEDLINE | ID: covidwho-1508355
ABSTRACT
BACKGROUND:
Emerging viruses like severe acute respiratory syndrome coronavirus (SARS-CoV), Crimean-Congo haemorrhagic fever virus (CCHFV) and Nipah virus (NiV) have been identified to pose a potential threat to transfusion safety. In this study, the ability of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate these viruses in platelet concentrates and plasma, respectively, was investigated. MATERIALS ANDMETHODS:
Blood products were spiked with SARS-CoV, CCHFV or NiV, and then treated with increasing doses of UVC light (THERAFLEX UV-Platelets) or with methylene blue (MB) plus increasing doses of visible light (MB/light; THERAFLEX MB-Plasma). Samples were taken before and after treatment with each illumination dose and tested for residual infectivity.RESULTS:
Treatment with half to three-fourths of the full UVC dose (0·2 J/cm2 ) reduced the infectivity of SARS-CoV (≥3·4 log), CCHFV (≥2·2 log) and NiV (≥4·3 log) to the limit of detection (LOD) in platelet concentrates, and treatment with MB and a fourth of the full light dose (120 J/cm2 ) decreased that of SARS-CoV (≥3·1 log), CCHFV (≥3·2 log) and NiV (≥2·7 log) to the LOD in plasma.CONCLUSION:
Our study demonstrates that both THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce the infectivity of SARS-CoV, CCHFV and NiV in platelet concentrates and plasma, respectively.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Ultraviolet Rays
/
Hemorrhagic Fever Virus, Crimean-Congo
/
Virus Inactivation
/
Severe acute respiratory syndrome-related coronavirus
/
Nipah Virus
/
Light
/
Methylene Blue
Type of study:
Experimental Studies
Limits:
Humans
Language:
English
Journal:
Vox Sang
Year:
2020
Document Type:
Article
Affiliation country:
Vox.12888
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