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Viral isolation analysis of SARS-CoV-2 from clinical specimens of COVID-19 patients.
Igarashi, Emiko; Tani, Hideki; Tamura, Kosuke; Itamochi, Masae; Shimada, Takahisa; Saga, Yumiko; Inasaki, Noriko; Hasegawa, Sumiyo; Yazawa, Shunsuke; Sasajima, Hitoshi; Kaya, Hiroyasu; Nomura, Satoshi; Itoh, Hiroyuki; Takeda, Shin-Ichi; Yamamoto, Yoshihiro; Yamashiro, Seiji; Ichikawa, Tomomi; Horie, Yukio; Hirota, Kojiro; Hirano, Norikazu; Kawashiri, Chikako; Oishi, Kazunori.
  • Igarashi E; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Tani H; Department of Virology, Toyama Institute of Health, Toyama, Japan. Electronic address: toyamaeiken3@juno.ocn.ne.jp.
  • Tamura K; Department of Research Planning, Toyama Institute of Health, Toyama, Japan.
  • Itamochi M; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Shimada T; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Saga Y; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Inasaki N; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Hasegawa S; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Yazawa S; Department of Virology, Toyama Institute of Health, Toyama, Japan.
  • Sasajima H; Department of Research Planning, Toyama Institute of Health, Toyama, Japan.
  • Kaya H; Toyama Prefectural Central Hospital, Toyama, Japan.
  • Nomura S; Toyama City Hospital, Toyama, Japan.
  • Itoh H; Takaoka City Hospital, Toyama, Japan.
  • Takeda SI; Kurobe City Hospital, Toyama, Japan.
  • Yamamoto Y; Toyama University Hospital, Toyama, Japan.
  • Yamashiro S; Toyama University Hospital, Toyama, Japan.
  • Ichikawa T; Toyama Red Cross Hospital, Toyama, Japan.
  • Horie Y; Saiseikai Toyama Hospital, Toyama, Japan.
  • Hirota K; Tonami General Hospital, Toyama, Japan.
  • Hirano N; Toyama Rosai Hospital, Toyama, Japan.
  • Kawashiri C; Toyama Institute of Health, Toyama, Japan.
  • Oishi K; Toyama Institute of Health, Toyama, Japan.
J Infect Chemother ; 28(2): 347-351, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-1510011
ABSTRACT
Genetic testing using reverse transcriptase real-time polymerase chain reaction (rRT-PCR) is the mainstay of diagnosis of COVID-19. However, it has not been fully investigated whether infectious viruses are contained in SARS-CoV-2 genome-positive specimens examined using the rRT-PCR test. In this study, we examined the correlation between the threshold Cycle (Ct) value obtained from the rRT-PCR test and virus isolation in cultured cells, using 533 consecutive clinical specimens of COVID-19 patients. The virus was isolated from specimens with a Ct value of less than 30 cycles, and the lower the Ct value, the more efficient the isolation rate. A cytopathic effect due to herpes simplex virus type 1 contamination was observed in one sample with a Ct value of 35 cycles. In a comparison of VeroE6/TMPRSS2 cells and VeroE6 cells used for virus isolation, VeroE6/TMPRSS2 cells isolated the virus 1.7 times more efficiently than VeroE6 cells. There was no significant difference between the two cells in the mean Ct value of the detectable sample. In conclusion, Lower Ct values in the PCR test were associated with higher virus isolation rates, and VeroE6/TMPRSS2 cells were able to isolate viruses more efficiently than VeroE6 cells.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Humans Language: English Journal: J Infect Chemother Journal subject: Microbiology / Drug Therapy Year: 2022 Document Type: Article Affiliation country: J.jiac.2021.10.028

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Limits: Humans Language: English Journal: J Infect Chemother Journal subject: Microbiology / Drug Therapy Year: 2022 Document Type: Article Affiliation country: J.jiac.2021.10.028