Duplex Reverse Transcription Multienzyme Isothermal Rapid Amplification Assays for Detecting SARS-CoV-2.
Clin Lab
; 67(11)2021 Nov 01.
Article
in English
| MEDLINE | ID: covidwho-1513105
ABSTRACT
BACKGROUND:
Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that brings a significant public health challenge. A rapid and simple method is necessary for testing suspected samples and screening the population.METHODS:
To better monitor sample effectiveness, this study described a method to detect nucleocapsid protein gene (N gene) of SARS-CoV-2 and human ACTB gene employing real-time duplex reverse transcription multienzyme isothermal rapid amplification (RT-MIRA) assays.RESULTS:
The established real-time duplex RT-MIRA assays showed that no cross-reactions were observed to other pathogens and the detection limit was 100 copies/reaction. Using simulated clinical samples to test established assays further and the amplification process took no more than 20 minutes at 42°C.CONCLUSIONS:
RT-MIRA assays are faster and easier than reverse transcription real-time polymerase chain reaction (RT-PCR). It is expected to be further optimized and evaluated in the detection of SARS-CoV-2 confirmed cases.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Reverse Transcription
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal subject:
Laboratory Techniques and procedures
Year:
2021
Document Type:
Article
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