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High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.
Fernandez, Marco A; Alzayat, Hammad; Jaimes, Maria C; Kharraz, Yacine; Requena, Gerard; Mendez, Pedro.
  • Fernandez MA; Flow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
  • Alzayat H; Flow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
  • Jaimes MC; Cytek Biosciences, Fremont, CA, USA.
  • Kharraz Y; Cytek Biosciences, Fremont, CA, USA.
  • Requena G; Flow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
  • Mendez P; Synthego Corporation, Menlo Park, CA, USA. pmendez00@gmail.com.
Methods Mol Biol ; 2386: 43-60, 2022.
Article in English | MEDLINE | ID: covidwho-1513947
ABSTRACT
A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunophenotyping / Flow Cytometry / COVID-19 Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-1771-7_4

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunophenotyping / Flow Cytometry / COVID-19 Limits: Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: 978-1-0716-1771-7_4