High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.
Methods Mol Biol
; 2386: 43-60, 2022.
Article
in English
| MEDLINE | ID: covidwho-1513947
ABSTRACT
A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Immunophenotyping
/
Flow Cytometry
/
COVID-19
Limits:
Humans
Language:
English
Journal:
Methods Mol Biol
Journal subject:
Molecular Biology
Year:
2022
Document Type:
Article
Affiliation country:
978-1-0716-1771-7_4
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