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Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2.
Huerga Encabo, Hector; Ulferts, Rachel; Sharma, Aneesh; Beale, Rupert; Bonnet, Dominique.
  • Huerga Encabo H; Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
  • Ulferts R; Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Sharma A; Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
  • Beale R; Cell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
  • Bonnet D; Hematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
STAR Protoc ; 2(4): 100903, 2021 12 17.
Article in English | MEDLINE | ID: covidwho-1514330
ABSTRACT
Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bone Marrow / Hematopoietic Stem Cells / Colony-Forming Units Assay / Fetal Blood / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100903

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bone Marrow / Hematopoietic Stem Cells / Colony-Forming Units Assay / Fetal Blood / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100903