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Real-time ultra-sensitive detection of SARS-CoV-2 by quasi-freestanding epitaxial graphene-based biosensor.
Kim, Soaram; Ryu, Heeju; Tai, Sheldon; Pedowitz, Michael; Rzasa, John Robertson; Pennachio, Daniel J; Hajzus, Jenifer R; Milton, Donald K; Myers-Ward, Rachael; Daniels, Kevin M.
  • Kim S; Department of Electrical and Computer Engineering, University of Maryland, College Park, MD, 20742, USA; Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, MD, 20742, USA. Electronic address: soaramk@umd.edu.
  • Ryu H; Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA.
  • Tai S; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, 20742, USA.
  • Pedowitz M; Department of Electrical and Computer Engineering, University of Maryland, College Park, MD, 20742, USA; Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, MD, 20742, USA.
  • Rzasa JR; Fischell Department of Bioengineering, University of Maryland, College Park, MD, 20742, USA.
  • Pennachio DJ; US Naval Research Laboratory, Washington, DC, 23075, USA.
  • Hajzus JR; US Naval Research Laboratory, Washington, DC, 23075, USA.
  • Milton DK; Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, College Park, MD, 20742, USA.
  • Myers-Ward R; US Naval Research Laboratory, Washington, DC, 23075, USA.
  • Daniels KM; Department of Electrical and Computer Engineering, University of Maryland, College Park, MD, 20742, USA; Institute for Research in Electronics and Applied Physics, University of Maryland, College Park, MD, 20742, USA.
Biosens Bioelectron ; 197: 113803, 2022 Feb 01.
Article in English | MEDLINE | ID: covidwho-1517063
ABSTRACT
We report the rapid detection of SARS-CoV-2 in infected patients (mid-turbinate swabs and exhaled breath aerosol samples) in concentrations as low as 60 copies/mL of the virus in seconds by electrical transduction of the SARS-CoV-2 S1 spike protein antigen via SARS-CoV-2 S1 spike protein antibodies immobilized on bilayer quasi-freestanding epitaxial graphene without gate or signal amplification. The sensor demonstrates the spike protein antigen detection in a concentration as low as 1 ag/mL. The heterostructure of the SARS-CoV-2 antibody/graphene-based sensor is developed through a simple and low-cost fabrication technique. Furthermore, sensors integrated into a portable testing unit distinguished B.1.1.7 variant positive samples from infected patients (mid-turbinate swabs and saliva samples, 4000-8000 copies/mL) with a response time of as fast as 0.6 s. The sensor is reusable, allowing for reimmobilization of the crosslinker and antibodies on the biosensor after desorption of biomarkers by NaCl solution or heat treatment above 40 °C.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 / Graphite Type of study: Diagnostic study Topics: Variants Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article