CRISPR-Cas12a-Based Detection of SARS-CoV-2 Harboring the E484K Mutation.
ACS Synth Biol
; 10(12): 3595-3599, 2021 12 17.
Article
in English
| MEDLINE | ID: covidwho-1517598
ABSTRACT
The novel respiratory virus SARS-CoV-2 is rapidly evolving across the world with the potential of increasing its transmission and the induced disease. Here, we applied the CRISPR-Cas12a system to detect, without the need of sequencing, SARS-CoV-2 genomes harboring the E484K mutation, first identified in the Beta variant and catalogued as an escape mutation. The E484K mutation creates a canonical protospacer adjacent motif for Cas12a recognition in the resulting DNA amplicon, which was exploited to obtain a differential readout. We analyzed a series of fecal samples from hospitalized patients in Valencia (Spain), finding one infection with SARS-CoV-2 harboring the E484K mutation, which was then confirmed by sequencing. Overall, these results suggest that CRISPR diagnostics can be a useful tool in epidemiology to monitor the spread of escape mutations.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
DNA, Viral
/
CRISPR-Cas Systems
/
COVID-19 Testing
/
SARS-CoV-2
/
COVID-19
/
Mutation
Type of study:
Diagnostic study
/
Observational study
/
Randomized controlled trials
Topics:
Variants
Limits:
Humans
Country/Region as subject:
Europa
Language:
English
Journal:
ACS Synth Biol
Year:
2021
Document Type:
Article
Affiliation country:
Acssynbio.1c00323
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