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Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics.
Sande, Maria G; Rodrigues, Joana L; Ferreira, Débora; Silva, Carla J; Rodrigues, Ligia R.
  • Sande MG; CEB-Centre of Biological Engineering, Campus de Gualtar, Universidade do Minho, 4710-057 Braga, Portugal.
  • Rodrigues JL; CEB-Centre of Biological Engineering, Campus de Gualtar, Universidade do Minho, 4710-057 Braga, Portugal.
  • Ferreira D; CEB-Centre of Biological Engineering, Campus de Gualtar, Universidade do Minho, 4710-057 Braga, Portugal.
  • Silva CJ; CENTI-Center for Nanotechnology and Smart Materials, Rua Fernando Mesquita 2785, 4760-034 Vila Nova de Famalicão, Portugal.
  • Rodrigues LR; CITEVE-Technological Center for the Textile and Clothing Industries of Portugal, Rua Fernando Mesquita 2785, 4760-034 Vila Nova de Famalicão, Portugal.
Biosensors (Basel) ; 11(11)2021 Oct 26.
Article in English | MEDLINE | ID: covidwho-1518595
ABSTRACT
Infectious agents, especially bacteria and viruses, account for a vast number of hospitalisations and mortality worldwide. Providing effective and timely diagnostics for the multiplicity of infectious diseases is challenging. Conventional diagnostic solutions, although technologically advanced, are highly complex and often inaccessible in resource-limited settings. An alternative strategy involves convenient rapid diagnostics which can be easily administered at the point-of-care (POC) and at low cost without sacrificing reliability. Biosensors and other rapid POC diagnostic tools which require biorecognition elements to precisely identify the causative pathogen are being developed. The effectiveness of these devices is highly dependent on their biorecognition capabilities. Naturally occurring biorecognition elements include antibodies, bacteriophages and enzymes. Recently, modified molecules such as DNAzymes, peptide nucleic acids and molecules which suffer a selective screening like aptamers and peptides are gaining interest for their biorecognition capabilities and other advantages over purely natural ones, such as robustness and lower production costs. Antimicrobials with a broad-spectrum activity against pathogens, such as antibiotics, are also used in dual diagnostic and therapeutic strategies. Other successful pathogen identification strategies use chemical ligands, molecularly imprinted polymers and Clustered Regularly Interspaced Short Palindromic Repeats-associated nuclease. Herein, the latest developments regarding biorecognition elements and strategies to use them in the design of new biosensors for pathogens detection are reviewed.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bacteria / Viruses / Biosensing Techniques / Point-of-Care Systems Type of study: Diagnostic study Language: English Year: 2021 Document Type: Article Affiliation country: Bios11110418

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Bacteria / Viruses / Biosensing Techniques / Point-of-Care Systems Type of study: Diagnostic study Language: English Year: 2021 Document Type: Article Affiliation country: Bios11110418