Evaluation of the Anti-Viral Activity of Human Recombinant Interferon Lambda-1 against SARS-CoV-2.
Bull Exp Biol Med
; 172(1): 53-56, 2021 Nov.
Article
in English
| MEDLINE | ID: covidwho-1520385
ABSTRACT
The antiviral activity of recombinant human IFN-lambda type 1 (IFNλ-1) against culture strain of SARS-CoV-2 virus was determined by infecting a highly sensitive VeroE6 coronavirus cell culture after preincubation test (the cell monolayer was incubated with 4-fold dilutions of IFNλ-1 in a concentration range of 0.16-42,500 ng/ml in a culture medium for 12 h at 37°C) and without preincubation (simultaneous addition of different concentrations of IFNλ-1 and SARS-CoV-2 infection in a dose of 102 TCID50). The created recombinant human IFNλ-1 demonstrated obvious antiviral activity against SARS-CoV-2 virus in vitro. In the tests with and without preincubation, IFNλ-1 exhibited significant activity, although somewhat lower in variant with simultaneous addition of IFNλ-1 and virus to the cell culture. It should be noted that the antiviral effect of IFNλ-1 was observed in a wide range of concentrations.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Antiviral Agents
/
Virus Replication
/
Recombinant Proteins
/
Interferons
/
Viral Load
/
SARS-CoV-2
Type of study:
Experimental Studies
Topics:
Variants
Limits:
Animals
/
Humans
Language:
English
Journal:
Bull Exp Biol Med
Year:
2021
Document Type:
Article
Affiliation country:
S10517-021-05330-0
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