Ultraprecise Antigen 10-in-1 Pool Testing by Multiantibodies Transistor Assay.
J Am Chem Soc
; 143(47): 19794-19801, 2021 12 01.
Article
in English
| MEDLINE | ID: covidwho-1521695
ABSTRACT
Effective screening of infectious diseases requires a fast, cheap, and population-scale testing. Antigen pool testing can increase the test rate and shorten the screening time, thus being a valuable approach for epidemic prevention and control. However, the overall percent agreement (OPA) with polymerase chain reaction (PCR) is one-half to three-quarters, hampering it from being a comprehensive method, especially pool testing, beyond the gold-standard PCR. Here, a multiantibodies transistor assay is developed for sensitive and highly precise antigen pool testing. The multiantibodies capture SARS-CoV-2 spike S1 proteins with different configurations, resulting in an antigen-binding affinity down to 0.34 fM. The limit of detection reaches 3.5 × 10-17 g mL-1SARS-CoV-2 spike S1 protein in artificial saliva, 4-5 orders of magnitude lower than existing transistor sensors. The testing of 60 nasopharyngeal swabs exhibits â¼100% OPA with PCR within an average diagnoses time of 38.9 s. Owing to its highly precise feature, a portable integrated platform is fabricated, which achieves 10-in-1 pooled screening for high testing throughput. This work solves the long-standing problem of antigen pool testing, enabling it to be a valuable tool in precise diagnoses and population-wide screening of COVID-19 or other epidemics in the future.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Transistors, Electronic
/
Immunoassay
/
Spike Glycoprotein, Coronavirus
/
Antibodies
Type of study:
Diagnostic study
Topics:
Long Covid
Language:
English
Journal:
J Am Chem Soc
Year:
2021
Document Type:
Article
Affiliation country:
Jacs.1c08598
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