SARS-CoV-2 infection and replication in human gastric organoids.
Nat Commun
; 12(1): 6610, 2021 11 16.
Article
in English
| MEDLINE | ID: covidwho-1521737
ABSTRACT
COVID-19 typically manifests as a respiratory illness, but several clinical reports have described gastrointestinal symptoms. This is particularly true in children in whom gastrointestinal symptoms are frequent and viral shedding outlasts viral clearance from the respiratory system. These observations raise the question of whether the virus can replicate within the stomach. Here we generate gastric organoids from fetal, pediatric, and adult biopsies as in vitro models of SARS-CoV-2 infection. To facilitate infection, we induce reverse polarity in the gastric organoids. We find that the pediatric and late fetal gastric organoids are susceptible to infection with SARS-CoV-2, while viral replication is significantly lower in undifferentiated organoids of early fetal and adult origin. We demonstrate that adult gastric organoids are more susceptible to infection following differentiation. We perform transcriptomic analysis to reveal a moderate innate antiviral response and a lack of differentially expressed genes belonging to the interferon family. Collectively, we show that the virus can efficiently infect the gastric epithelium, suggesting that the stomach might have an active role in fecal-oral SARS-CoV-2 transmission.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Stomach
/
Virus Replication
/
Organoids
/
SARS-CoV-2
/
COVID-19
/
Intestinal Mucosa
Type of study:
Observational study
/
Prognostic study
Topics:
Long Covid
Limits:
Aged
/
Animals
/
Child
/
Child, preschool
/
Humans
/
Infant
/
Middle aged
Language:
English
Journal:
Nat Commun
Journal subject:
Biology
/
Science
Year:
2021
Document Type:
Article
Affiliation country:
S41467-021-26762-2
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