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NK and T Cell Immunological Signatures in Hospitalized Patients with COVID-19.
Bergantini, Laura; d'Alessandro, Miriana; Cameli, Paolo; Cavallaro, Dalila; Gangi, Sara; Cekorja, Behar; Sestini, Piersante; Bargagli, Elena.
  • Bergantini L; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • d'Alessandro M; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • Cameli P; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • Cavallaro D; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • Gangi S; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • Cekorja B; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • Sestini P; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
  • Bargagli E; Respiratory Diseases Unit, Department of Medical Sciences, University Hospital of Siena (Azienda Ospedaliera Universitaria Senese. AOUS), Viale Bracci, 53100 Siena, Italy.
Cells ; 10(11)2021 11 15.
Article in English | MEDLINE | ID: covidwho-1523882
ABSTRACT
Severe acute respiratory syndrome caused by coronavirus 2 emerged in Wuhan (China) in December 2019 and has severely challenged the human population. NK and T cells are involved in the progression of COVID-19 infection through the ability of NK cells to modulate T-cell responses, and by the stimulation of cytokine release. No detailed investigation of the NK cell landscape in clinical SARS-CoV-2 infection has yet been reported. A total of 35 COVID-19 hospitalised patients were stratified for clinical severity and 17 healthy subjects were enrolled. NK cell subsets and T cell subsets were analysed with flow cytometry. Serum cytokines were detected with a bead-based multiplex assay. Fewer CD56dimCD16brightNKG2A+NK cells and a parallel increase in the CD56+CD69+NK, CD56+PD-1+NK, CD56+NKp44+NK subset were reported in COVID-19 than HC. A significantly higher adaptive/memory-like NK cell frequency in patients with severe disease than in those with mild and moderate phenotypes were reported. Moreover, adaptive/memory-like NK cell frequencies were significantly higher in patients who died than in survivors. Severe COVID-19 patients showed higher serum concentrations of IL-6 than mild and control groups. Direct correlation emerged for IL-6 and adaptive/memory-like NK. All these findings provide new insights into the immune response of patients with COVID-19. In particular, they demonstrate activation of NK through overexpression of CD69 and CD25 and show that PD-1 inhibitory signalling maintains an exhausted phenotype in NK cells. These results suggest that adaptive/memory-like NK cells could be the basis of promising targeted therapy for future viral infections.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Killer Cells, Natural / T-Lymphocytes / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Limits: Aged / Female / Humans / Male / Middle aged Language: English Year: 2021 Document Type: Article Affiliation country: Cells10113182

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Killer Cells, Natural / T-Lymphocytes / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Randomized controlled trials Limits: Aged / Female / Humans / Male / Middle aged Language: English Year: 2021 Document Type: Article Affiliation country: Cells10113182