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Nanoparticle encapsulation of non-genotoxic p53 activator Inauhzin-C for improved therapeutic efficacy.
Bhattarai, Nimisha; Wang, Jieqiong; Nguyen, Daniel; Yang, Xiaoxiao; Helmers, Linh; Paruch, Jennifer; Li, Li; Zhang, Yiwei; Meng, Kun; Wang, Alun; Jayawickramarajah, Janarthanan; Wang, Binghe; Zeng, Shelya; Lu, Hua.
  • Bhattarai N; Department of Biochemistry & Molecular Biology and Cancer Center, Tulane University School of Medicine, New Orleans, LA, USA.
  • Wang J; Department of Biochemistry & Molecular Biology and Cancer Center, Tulane University School of Medicine, New Orleans, LA, USA.
  • Nguyen D; Department of Biochemistry & Molecular Biology and Cancer Center, Tulane University School of Medicine, New Orleans, LA, USA.
  • Yang X; Department of Chemistry, Georgia State University, Atlanta, GA, USA.
  • Helmers L; Laboratory of Cellular Immunology, Ochsner Clinic Foundation, New Orleans, LA 70121, USA.
  • Paruch J; Laboratory of Cellular Immunology, Ochsner Clinic Foundation, New Orleans, LA 70121, USA.
  • Li L; Laboratory of Cellular Immunology, Ochsner Clinic Foundation, New Orleans, LA 70121, USA.
  • Zhang Y; Department of Biochemistry & Molecular Biology and Cancer Center, Tulane University School of Medicine, New Orleans, LA, USA.
  • Meng K; Department of Chemistry, Tulane University School of Science and Engineering, New Orleans, LA, USA.
  • Wang A; Department of Pathology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Jayawickramarajah J; Department of Chemistry, Tulane University School of Science and Engineering, New Orleans, LA, USA.
  • Wang B; Department of Chemistry, Georgia State University, Atlanta, GA, USA.
  • Zeng S; Department of Biochemistry & Molecular Biology and Cancer Center, Tulane University School of Medicine, New Orleans, LA, USA.
  • Lu H; Department of Biochemistry & Molecular Biology and Cancer Center, Tulane University School of Medicine, New Orleans, LA, USA.
Theranostics ; 11(14): 7005-7017, 2021.
Article in English | MEDLINE | ID: covidwho-1524524
ABSTRACT
The tumor suppressor protein p53 remains in a wild type but inactive form in ~50% of all human cancers. Thus, activating it becomes an attractive approach for targeted cancer therapies. In this regard, our lab has previously discovered a small molecule, Inauhzin (INZ), as a potent p53 activator with no genotoxicity.

Method:

To improve its efficacy and bioavailability, here we employed nanoparticle encapsulation, making INZ-C, an analog of INZ, to nanoparticle-encapsulated INZ-C (n-INZ-C).

Results:

This approach significantly improved p53 activation and inhibition of lung and colorectal cancer cell growth by n-INZ-C in vitro and in vivo while it displayed a minimal effect on normal human Wi38 and mouse MEF cells. The improved activity was further corroborated with the enhanced cellular uptake observed in cancer cells and minimal cellular uptake observed in normal cells. In vivo pharmacokinetic evaluation of these nanoparticles showed that the nanoparticle encapsulation prolongates the half-life of INZ-C from 2.5 h to 5 h in mice.

Conclusions:

These results demonstrate that we have established a nanoparticle system that could enhance the bioavailability and efficacy of INZ-C as a potential anti-cancer therapeutic.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Phenothiazines / Colorectal Neoplasms / Tumor Suppressor Protein p53 / Nanoparticles / Indoles / Lung Neoplasms / Antineoplastic Agents Type of study: Experimental Studies Limits: Animals / Humans Language: English Journal: Theranostics Year: 2021 Document Type: Article Affiliation country: Thno.57404

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Phenothiazines / Colorectal Neoplasms / Tumor Suppressor Protein p53 / Nanoparticles / Indoles / Lung Neoplasms / Antineoplastic Agents Type of study: Experimental Studies Limits: Animals / Humans Language: English Journal: Theranostics Year: 2021 Document Type: Article Affiliation country: Thno.57404