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Carbon nanotube field-effect transistor (CNT-FET)-based biosensor for rapid detection of SARS-CoV-2 (COVID-19) surface spike protein S1.
Zamzami, Mazin A; Rabbani, Gulam; Ahmad, Abrar; Basalah, Ahmad A; Al-Sabban, Wesam H; Nate Ahn, Saeyoung; Choudhry, Hani.
  • Zamzami MA; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Centre of Artificial Intelligence in Precision Medicines, King Abdulaziz University
  • Rabbani G; Nano Diagnostics & Devices (NDD), IT Medical Fusion Center, 350-27 Gumidae-ro, Gumi-si, Gyeongbuk 39253, Republic of Korea.
  • Ahmad A; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Basalah AA; Department of Mechanical Engineering, College of Engineering & Islamic Architecture, Umm Al-Qura University, Makkah, Saudi Arabia.
  • Al-Sabban WH; Department of Information Systems, College of Computer and Information Systems, Umm Al-Qura University, Makkah, Saudi Arabia.
  • Nate Ahn S; Nano Diagnostics & Devices (NDD), IT Medical Fusion Center, 350-27 Gumidae-ro, Gumi-si, Gyeongbuk 39253, Republic of Korea; Fuzbien Technology Institute, 13 Taft Court, suite 222, Rockville, MD 20850, USA. Electronic address: nate.ahn@gmail.com.
  • Choudhry H; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Centre of Artificial Intelligence in Precision Medicines, King Abdulaziz University
Bioelectrochemistry ; 143: 107982, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-1525699
ABSTRACT
The large-scale diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for traceability and treatment during pandemic outbreaks. We developed a fast (2-3 min), easy-to-use, low-cost, and quantitative electrochemical biosensor based on carbon nanotube field-effect transistor (CNT-FET) that allows digital detection of the SARS-CoV-2 S1 in fortifited saliva samples for quick and accurate detection of SARS-CoV-2 S1 antigens. The biosensor was developed on a Si/SiO2 surface by CNT printing with the immobilization of a anti-SARS-CoV-2 S1. SARS-CoV-2 S1 antibody was immobilized on the CNT surface between the S-D channel area using a linker 1-pyrenebutanoic acid succinimidyl ester (PBASE) through non-covalent interaction. A commercial SARS-CoV-2 S1 antigen was used to characterize the electrical output of the CNT-FET biosensor. The SARS-CoV-2 S1 antigen in the 10 mM AA buffer pH 6.0 was effectively detected by the CNT-FET biosensor at concentrations from 0.1 fg/mL to 5.0 pg/mL. The limit of detection (LOD) of the developed CNT-FET biosensor was 4.12 fg/mL. The selectivity test was performed by using target SARS-CoV-2 S1 and non-target SARS-CoV-1 S1 and MERS-CoV S1 antigens in the 10 mM AA buffer pH 6.0. The biosensor showed high selectivity (no response to SARS-CoV-1 S1 or MERS-CoV S1 antigen) with SARS-CoV-2 S1 antigen detection in the 10 mM AA buffer pH 6.0. The biosensor is highly sensitive, saves time, and could be a helpful platform for rapid detection of SARS-CoV-2 S1 antigen from the patients saliva.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanotubes, Carbon / Electrochemical Techniques / Spike Glycoprotein, Coronavirus / SARS-CoV-2 Type of study: Diagnostic study / Experimental Studies Language: English Journal: Bioelectrochemistry Journal subject: Biochemistry Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nanotubes, Carbon / Electrochemical Techniques / Spike Glycoprotein, Coronavirus / SARS-CoV-2 Type of study: Diagnostic study / Experimental Studies Language: English Journal: Bioelectrochemistry Journal subject: Biochemistry Year: 2022 Document Type: Article