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Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV-2 replication.
Jiang, Yiling; Tong, Kuijie; Yao, Roubin; Zhou, Yuanze; Lin, Hanwen; Du, Liubing; Jin, Yunyun; Cao, Liu; Tan, Jingquan; Zhang, Xing-Ding; Guo, Deyin; Pan, Ji-An; Peng, Xiaoxue.
  • Jiang Y; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Tong K; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Yao R; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Zhou Y; Nanjing CRYCISION Biotechnology Co., Ltd, Nanjing, 211100, China.
  • Lin H; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Du L; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Jin Y; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Cao L; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Tan J; Nanjing CRYCISION Biotechnology Co., Ltd, Nanjing, 211100, China.
  • Zhang XD; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Guo D; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China.
  • Pan JA; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China. panjan@mail.sysu.edu.cn.
  • Peng X; The Center for Infection and Immunity Study and Molecular Cancer Research Center, School of Medicine, Sun Yat-Sen University, Guangming Science City, Shenzhen, 518107, China. pengxx9@mail.sysu.edu.cn.
Cell Biosci ; 11(1): 140, 2021 Jul 22.
Article in English | MEDLINE | ID: covidwho-1526659
ABSTRACT

BACKGROUND:

Analysis of viral protein-protein interactions is an essential step to uncover the viral protein functions and the molecular mechanism for the assembly of a viral protein complex. We employed a mammalian two-hybrid system to screen all the viral proteins of SARS-CoV-2 for the protein-protein interactions.

RESULTS:

Our study detected 48 interactions, 14 of which were firstly reported here. Unlike Nsp1 of SARS-CoV, Nsp1 of SARS-CoV-2 has the most interacting partners among all the viral proteins and likely functions as a hub for the viral proteins. Five self-interactions were confirmed, and five interactions, Nsp1/Nsp3.1, Nsp3.1/N, Nsp3.2/Nsp12, Nsp10/Nsp14, and Nsp10/Nsp16, were determined to be positive bidirectionally. Using the replicon reporter system of SARS-CoV-2, we screened all viral Nsps for their impacts on the viral replication and revealed Nsp3.1, the N-terminus of Nsp3, significantly inhibited the replicon reporter gene expression. We found Nsp3 interacted with N through its acidic region at N-terminus, while N interacted with Nsp3 through its NTD, which is rich in the basic amino acids. Furthermore, using purified truncated N and Nsp3 proteins, we determined the direct interactions between Nsp3 and N protein.

CONCLUSIONS:

Our findings provided a basis for understanding the functions of coronavirus proteins and supported the potential of interactions as the target for antiviral drug development.
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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Cell Biosci Year: 2021 Document Type: Article Affiliation country: S13578-021-00644-y

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: Cell Biosci Year: 2021 Document Type: Article Affiliation country: S13578-021-00644-y