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A proof-of-concept of parallel single-drop microextraction for the rapid and sensitive biomonitoring of pesticides in urine.
Mafra, Gabriela; Will, Camila; Huelsmann, Ricardo; Merib, Josias; Carasek, Eduardo.
  • Mafra G; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, South Carolina, 88040-900, Brazil.
  • Will C; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, South Carolina, 88040-900, Brazil.
  • Huelsmann R; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, South Carolina, 88040-900, Brazil.
  • Merib J; Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Rio Grande do Sul, 90050-170, Brazil.
  • Carasek E; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, South Carolina, 88040-900, Brazil.
J Sep Sci ; 44(9): 1961-1968, 2021 May.
Article in English | MEDLINE | ID: covidwho-1527448
Semantic information from SemMedBD (by NLM)
1. Urine - SpecimenType PART_OF Homo sapiens
Subject
Urine - SpecimenType
Predicate
PART_OF
Object
Homo sapiens
2. Urine - SpecimenType LOCATION_OF Dichlorodiphenyl Dichloroethylene
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
Dichlorodiphenyl Dichloroethylene
3. Urine - SpecimenType LOCATION_OF DDT (Insecticide)
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
DDT (Insecticide)
4. Urine - SpecimenType LOCATION_OF pendimethalin
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
pendimethalin
5. Urine - SpecimenType LOCATION_OF tebuconazole
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
tebuconazole
6. Study MEASURES Dichlorodiphenyl Dichloroethylene
Subject
Study
Predicate
MEASURES
Object
Dichlorodiphenyl Dichloroethylene
7. Study MEASURES DDT (Insecticide)
Subject
Study
Predicate
MEASURES
Object
DDT (Insecticide)
8. Study MEASURES pendimethalin
Subject
Study
Predicate
MEASURES
Object
pendimethalin
9. Study MEASURES tebuconazole
Subject
Study
Predicate
MEASURES
Object
tebuconazole
10. Urine - SpecimenType PART_OF Homo sapiens
Subject
Urine - SpecimenType
Predicate
PART_OF
Object
Homo sapiens
11. Urine - SpecimenType LOCATION_OF Dichlorodiphenyl Dichloroethylene
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
Dichlorodiphenyl Dichloroethylene
12. Urine - SpecimenType LOCATION_OF DDT (Insecticide)
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
DDT (Insecticide)
13. Urine - SpecimenType LOCATION_OF pendimethalin
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
pendimethalin
14. Urine - SpecimenType LOCATION_OF tebuconazole
Subject
Urine - SpecimenType
Predicate
LOCATION_OF
Object
tebuconazole
15. Study MEASURES Dichlorodiphenyl Dichloroethylene
Subject
Study
Predicate
MEASURES
Object
Dichlorodiphenyl Dichloroethylene
16. Study MEASURES DDT (Insecticide)
Subject
Study
Predicate
MEASURES
Object
DDT (Insecticide)
17. Study MEASURES pendimethalin
Subject
Study
Predicate
MEASURES
Object
pendimethalin
18. Study MEASURES tebuconazole
Subject
Study
Predicate
MEASURES
Object
tebuconazole
ABSTRACT
In this study, a lab-made parallel single-drop microextraction methodology using the magnetic ionic liquid trihexyltetradecylphosphonium tetrachloromanganate (II) as extraction solvent was developed to determine the pesticides tebuconazole, pendimethalin, dichlorodiphenyltrichloroethane, and dichlorodiphenyldichloroethylene in human urine samples. The experimental setup consisted of a 96-well plate system containing a set of magnetic pins that allowed for the manipulation of up to 96 samples simultaneously, providing an enhanced drop stability compared to traditional single-drop microextraction approaches. The optimal conditions employed 5.38 ± 0.55 mg of extraction solvent, 1.5 mL of diluted urine samples (110), extraction time of 130 min, and subsequent dilution in 20 µL of acetonitrile. The method exhibited satisfactory analytical performance, with limits of detection of 7.5 µg/L for all analytes and coefficients of determination higher than 0.9955. Intraday and interday precisions ranged from 3 to 17% (n = 3) and 15 to 18% (n = 9), respectively, with relative recovery of analytes ranging from 70 to 122%. The method proposed was successfully applied in two human urine samples and no sign of the analytes was detected. The results demonstrated that the proposed method allowed for cost-effective and high-throughput methodology to be explored as a valuable tool in bioanalytical applications.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pesticides / Liquid Phase Microextraction / Biological Monitoring Type of study: Diagnostic study Limits: Humans Language: English Journal: J Sep Sci Year: 2021 Document Type: Article Affiliation country: Jssc.202001157

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pesticides / Liquid Phase Microextraction / Biological Monitoring Type of study: Diagnostic study Limits: Humans Language: English Journal: J Sep Sci Year: 2021 Document Type: Article Affiliation country: Jssc.202001157