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Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection.
Tagarro, Alfredo; Sanz-Santaeufemia, Francisco José; Grasa, Carlos; Cobos, Elena; Yebra, Julia; Alonso-Cadenas, Jose Antonio; Baquero-Artigao, Fernando; Mesa-Guzmán, Juan Miguel; Pérez-Seoane, Beatriz; Calvo, Cristina; Herreros, María Luisa; Epalza, Cristina; Melendo, Susana; Dominguez-Rodriguez, Sara; Vidal, Paula; Pacheco, Mónica; Ballesteros, Alvaro; Bernardino, María; Villanueva-Medina, Sara; Rodríguez-Molino, Paula; Miragaya Castro, Sandra; Rivière, Jacques; Garcés, Rosa; Santiago, Begoña; Fumadó, Victoria; Urretavizcaya-Martínez, María; García-García, María Luz; Penín, María; Cava, Fernando; Sáez, Elena; Iglesias-Bouzas, María Isabel; Herrero, Blanca; Reinoso, Teresa de Jesús; Moraleda, Cinta.
  • Tagarro A; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain; Pediatrics Research Group, Europea University of Madrid, Madrid, Spain; Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spai
  • Sanz-Santaeufemia FJ; Pediatrics Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Grasa C; Pediatrics, Infectious and Tropical Diseases Department, Hospital Universitario La Paz, Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain.
  • Cobos E; Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain.
  • Yebra J; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain.
  • Alonso-Cadenas JA; Emergency Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Baquero-Artigao F; Pediatrics, Infectious and Tropical Diseases Department, Hospital Universitario La Paz, Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain.
  • Mesa-Guzmán JM; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain.
  • Pérez-Seoane B; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain.
  • Calvo C; Pediatrics, Infectious and Tropical Diseases Department, Hospital Universitario La Paz, Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain.
  • Herreros ML; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain.
  • Epalza C; Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain; Pediatrics Department, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Melendo S; Infectious Diseases and Pediatric Immunology Unit, Department of Pediatrics, Hospital Universitario Vall d'Hebron, Madrid, Spain.
  • Dominguez-Rodriguez S; Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain.
  • Vidal P; Pediatrics Department, Hospital Clínico Universitario Lozano Blesa Zaragoza, Aragón, Spain.
  • Pacheco M; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain.
  • Ballesteros A; Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain.
  • Bernardino M; Pediatrics Research Group, Europea University of Madrid, Madrid, Spain.
  • Villanueva-Medina S; Pediatrics Department, Hospital Universitario 12 de Octubre, Madrid, Spain.
  • Rodríguez-Molino P; Pediatrics, Infectious and Tropical Diseases Department, Hospital Universitario La Paz, Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain.
  • Miragaya Castro S; Emergency Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Rivière J; Infectious Diseases and Pediatric Immunology Unit, Department of Pediatrics, Hospital Universitario Vall d'Hebron, Madrid, Spain.
  • Garcés R; Pediatrics Department, Hospital Clínico Universitario Lozano Blesa Zaragoza, Aragón, Spain.
  • Santiago B; Pediatric Infectious Diseases Unit. Department of Pediatrics, Hospital Universitario Gregorio Marañón, Madrid, Spain.
  • Fumadó V; Pediatric Infectious Diseases Unit, Department of Pediatrics, Hospital Universitario Sant Joan de Deu Barcelona, Barcelona, Spain.
  • Urretavizcaya-Martínez M; Pediatrics Department, Complejo Hospitalario de Navarra, Pamplona, Spain.
  • García-García ML; Pediatrics Department, Hospital Universitario Severo Ochoa Madrid, Madrid, Spain.
  • Penín M; Pediatrics Departament, Hospital Universitario Príncipe de Asturias, Madrid, Spain.
  • Cava F; Microbiology Department, UR Salud, Hospital Universitario Infanta Sofía, Madrid, Spain.
  • Sáez E; Microbiology Department, UR Salud, Hospital Universitario Infanta Sofía, Madrid, Spain.
  • Iglesias-Bouzas MI; Pediatric Intensive Care Unit, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Herrero B; Oncology Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain.
  • Reinoso TJ; Pediatrics Department, Hospital Universitario Infanta Sofía, San Sebastián de los Reyes, Madrid, Spain. Electronic address: treinosol@gmail.com.
  • Moraleda C; Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain; Pediatrics Department, Hospital Universitario 12 de Octubre, Madrid, Spain.
J Pediatr ; 2021 Sep 25.
Article in English | MEDLINE | ID: covidwho-1527773
ABSTRACT

OBJECTIVES:

To determine the time to reverse transcription-polymerase chain reaction (RT-PCR) negativity after the first positive RT-PCR test, factors associated with longer time to RT-PCR negativity, proportion of children seroconverting after proven severe acute respiratory syndrome coronavirus 2 infection, and factors associated with the lack of seroconversion. STUDY

DESIGN:

The Epidemiological Study of Coronavirus in Children of the Spanish Society of Pediatrics is a multicenter study conducted in Spanish children to assess the characteristics of coronavirus disease 2019. In a subset of patients, 3 serial RT-PCR tests on nasopharyngeal swab specimens were performed after the first RT-PCR test, and immunoglobulin G serology for severe acute respiratory syndrome coronavirus 2 antibodies was performed in the acute and follow-up (<14 and ≥14 days after diagnosis) phase.

RESULTS:

In total, 324 patients were included in the study. The median time to RT-PCR negativity was 17 days (IQR, 8-29 days), and 35% of patients remained positive more than 4 weeks after the first RT-PCR test. The probability of RT-PCR negativity did not differ across groups defined by sex, disease severity, immunosuppressive drugs, or clinical phenotype. Globally, 24% of children failed to seroconvert after infection. Seroconversion was associated with hospitalization, persistence of RT-PCR positivity, and days of fever.

CONCLUSIONS:

Time to RT-PCR negativity was long, regardless of the severity of symptoms or other patient features. This finding should be considered when interpreting RT-PCR results in a child with symptoms, especially those with mild symptoms. Seroprevalence and postimmunization studies should consider that 11 in 4 infected children fail to seroconvert.
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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Language: English Clinical aspect: Prognosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Language: English Clinical aspect: Prognosis Year: 2021
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