Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts.
Bioengineering (Basel)
; 8(11)2021 Nov 13.
Article
in English
| MEDLINE | ID: covidwho-1533772
ABSTRACT
Heat inactivation of bovine sera is routinely performed in cell culture laboratories. Nevertheless, it remains debatable whether it is still necessary due to the improvement of the production process of bovine sera. Do the benefits balance the loss of many proteins, such as hormones and growth factors, that are very useful for cell culture? This is even truer in the case of tissue engineering, the processes of which is often very demanding. This balance is examined here, from nine populations of fibroblasts originating from three different organs, by comparing the capacity of adhesion and proliferation of cells, their metabolism, and the capacity to produce the stroma; their histological appearance, thickness, and mechanical properties were also evaluated. Overall, serum inactivation does not appear to provide a significant benefit.
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International databases
Database:
MEDLINE
Type of study:
Experimental Studies
Language:
English
Year:
2021
Document Type:
Article
Affiliation country:
Bioengineering8110184
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