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Antibody titers against the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2 induced by BNT162b2 vaccination measured using automated chemiluminescent enzyme immunoassay.
Kato, Hideaki; Miyakawa, Kei; Ohtake, Norihisa; Go, Hirofumi; Yamaoka, Yutaro; Yajima, Satoshi; Shimada, Tomoko; Goto, Atsushi; Nakajima, Hideaki; Ryo, Akihide.
  • Kato H; Infection Prevention and Control Department, Yokohama City University Hospital, Yokohama, Japan; Department of Hematology and Clinical Immunology, Yokohama City University School of Medicine, Yokohama, Japan.
  • Miyakawa K; Department of Microbiology, Yokohama City University School of Medicine, Yokohama, Japan.
  • Ohtake N; Bioscience Division, Research and Development Department, Tosoh Corporation, Tokyo Research Center, Kanagawa, Japan; Advanced Medical Research Center, Yokohama City University, Yokohama, Japan.
  • Go H; Department of Biostatistics, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
  • Yamaoka Y; Department of Microbiology, Yokohama City University School of Medicine, Yokohama, Japan; Life Science Laboratory, Technology and Development Division, Kanto Chemical Co, Inc., Isehara, Japan.
  • Yajima S; Clinical Laboratory Department, Yokohama City University Hospital, Yokohama, Japan.
  • Shimada T; Nursing Department, Yokohama City University Hospital, Yokohama, Japan.
  • Goto A; Department of Health Data Science, Yokohama City University Graduate School of Data Science, Yokohama, Japan.
  • Nakajima H; Department of Hematology and Clinical Immunology, Yokohama City University School of Medicine, Yokohama, Japan.
  • Ryo A; Department of Microbiology, Yokohama City University School of Medicine, Yokohama, Japan. Electronic address: aryo@yokohama-cu.ac.jp.
J Infect Chemother ; 28(2): 273-278, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-1536654
Preprint
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ABSTRACT

BACKGROUND:

Levels of 50% neutralizing titer (NT50) reflect the a vaccine-induced humoral immunity after the vaccination against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Measurements of NT50 are difficult to implement in large quantities. A high-throughput laboratory test is expected for determining the level of herd immunity against SARS-CoV-2.

METHODS:

We analyzed samples from 168 Japanese healthcare workers who had completed two doses of the BNT162b2 vaccine. We analyzed immunoglobulin G (IgG) index values against spike protein (SP) using automated chemiluminescent enzyme immunoassay system AIA-CL and analyzed the background factors affecting antibody titer. SP IgG index was compared with 50% neutralization titers.

RESULTS:

The median SP IgG index values of the subjects (mean age = 43 years; 75% female) were 0.1, 1.35, 60.80, and 97.35 before and at 2, 4, and 6 weeks after the first dose, respectively. At 4 and 6 weeks after the first dose, SP IgG titers were found to have positive correlation with NT50 titer (r = 0.7535 in 4 weeks; r = 0.4376 in 6 weeks). Proportions of the SP IgG index values against the Alpha, Beta, Gamma, and Delta variants compared with the original strain were 2.029, 0.544, 1.017, and 0.6096 respectively. Older age was associated with lower SP IgG titer index 6 weeks after the first dose.

CONCLUSIONS:

SP IgG index values were rised at 3 weeks after two doses of BNT162b2 vaccination and have positive correlation with NT50. SP IgG index values were lower in the older individuals and against Beta and Delta strain.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Limits: Adult / Aged / Female / Humans / Male Language: English Journal: J Infect Chemother Journal subject: Microbiology / Drug Therapy Year: 2022 Document Type: Article Affiliation country: J.jiac.2021.11.021

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Limits: Adult / Aged / Female / Humans / Male Language: English Journal: J Infect Chemother Journal subject: Microbiology / Drug Therapy Year: 2022 Document Type: Article Affiliation country: J.jiac.2021.11.021