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Alternative SARS-CoV-2 detection protocol from self-collected saliva for mass diagnosis and epidemiological studies in low-incoming regions.
de Oliveira, Luana Prado Rolim; Cabral, Aline Diniz; Dos Santos Carmo, Andreia Moreira; Duran, Adriana Feliciano; Fermino, Diego Marin; Veiga, Glaucia Raquel Luciano; da Costa Aguiar Alves, Beatriz; Santana, Carla Moreira; Garcia, Felipe Baena; Santos, Edmar Silva; Jordão, Felipe Trovalim; Siqueira, Andressa Moreira; de Campos, Ivana Barros; Colpas, Daniela Rodrigues; Almeida, Fernanda Nascimento; Fonseca, Fernando Luiz Affonso; Sperança, Márcia Aparecida.
  • de Oliveira LPR; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Cabral AD; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Dos Santos Carmo AM; Santo André Regional Center, Adolfo Lutz Institute (ALI), Santo André, São Paulo, Brazil.
  • Duran AF; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Fermino DM; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Veiga GRL; Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André, São Paulo, Brazil.
  • da Costa Aguiar Alves B; Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André, São Paulo, Brazil.
  • Santana CM; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Garcia FB; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Santos ES; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Jordão FT; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • Siqueira AM; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil.
  • de Campos IB; Santo André Regional Center, Adolfo Lutz Institute (ALI), Santo André, São Paulo, Brazil.
  • Colpas DR; Santo André Regional Center, Adolfo Lutz Institute (ALI), Santo André, São Paulo, Brazil.
  • Almeida FN; Centro de Engenharia, Modelagem e Ciências Sociais Aplicadas, Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil.
  • Fonseca FLA; Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André, São Paulo, Brazil; Departamento de Ciências Farmacêuticas, Universidade Federal de São Paulo (UNIFESP), Diadema, São Paulo, Brazil.
  • Sperança MA; Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo, São Paulo, Brazil. Electronic address: marcia.speranca@ufabc.edu.br.
J Virol Methods ; 300: 114382, 2022 02.
Article in English | MEDLINE | ID: covidwho-1536941
ABSTRACT
Until mass vaccination befalls, control of the new betacoronavirus-associated severe acute respiratory syndrome pandemic (SARS-CoV-2) is based on decreasing virus circulation by social distancing and blocking transmission foci after diagnosis. Globally adopted SARS-CoV-2 diagnostic criteria embrace viral RNA detection by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) on nasopharynx secretions, which requires healthcare facilities and specialized personnel for sample collection. To develop an alternative protocol, hydrophilic cotton as the material and saliva as the source for biological sample collection in qRT-PCR/RT-endpoint-PCR SARS-CoV-2 diagnostic methods prepared with local consumables were evaluated using 99 archived nasopharynx samples previously diagnosed as positive for SARS-CoV-2 and 111 prospective saliva samples pared with nasopharynx samples from patients attending the local reference ABC Medical School diagnostic laboratory. The kappa agreement coefficient between the SARS-CoV-2 qRT-PCR and RT-endpoint-PCR was k = 0.97 (95 % CI 0.92-1.00) and k = 0.90 (95 % CI 0.81-0.99), respectively, on SARS-CoV-2-positive archived samples, with the initial qRT-PCR CT under 25. The agreement coefficient of the SARS-CoV-2 alternative saliva diagnostic protocol, when used to test the paired nasopharynx samples, was k = 0.79 (95 % CI 0.56-1,00). These data support that the SARS-CoV-2 diagnostic assay based on self-collected saliva on cotton represents an alternative protocol for mass diagnosis and epidemiological studies in low-income regions.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: J Virol Methods Year: 2022 Document Type: Article Affiliation country: J.jviromet.2021.114382

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Cohort study / Experimental Studies / Observational study / Prognostic study Topics: Vaccines Limits: Humans Language: English Journal: J Virol Methods Year: 2022 Document Type: Article Affiliation country: J.jviromet.2021.114382