Your browser doesn't support javascript.
Binding of phosphatidylserine-positive microparticles by PBMCs classifies disease severity in COVID-19 patients.
Rausch, Lisa; Lutz, Konstantin; Schifferer, Martina; Winheim, Elena; Gruber, Rudi; Oesterhaus, Elina F; Rinke, Linus; Hellmuth, Johannes C; Scherer, Clemens; Muenchhoff, Maximilian; Mandel, Christopher; Bergwelt-Baildon, Michael; Simons, Mikael; Straub, Tobias; Krug, Anne B; Kranich, Jan; Brocker, Thomas.
  • Rausch L; Institute for Immunology, Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Munich, Germany.
  • Lutz K; Institute for Immunology, Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Munich, Germany.
  • Schifferer M; German Center for Neurodegenerative Diseases (DZNE), Munich, Germany.
  • Winheim E; Munich Cluster of Systems Neurology (Synergy), Munich, Germany.
  • Gruber R; Institute for Immunology, Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Munich, Germany.
  • Oesterhaus EF; bene pharmaChem GmbH & Co.KG., Geretsried, Germany.
  • Rinke L; COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany.
  • Hellmuth JC; Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
  • Scherer C; Institute for Immunology, Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Munich, Germany.
  • Muenchhoff M; COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany.
  • Mandel C; Department of Medicine III, University Hospital, LMU Munich, Munich, Germany.
  • Bergwelt-Baildon M; COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany.
  • Simons M; Department of Medicine I, University Hospital, LMU Munich, Munich, Germany.
  • Straub T; COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany.
  • Krug AB; Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
  • Kranich J; German Center for Infection Research (DZIF), partner site Munich, Germany.
  • Brocker T; COVID-19 Registry of the LMU Munich (CORKUM), University Hospital, LMU Munich, Munich, Germany.
J Extracell Vesicles ; 10(14): e12173, 2021 12.
Article in English | MEDLINE | ID: covidwho-1544291
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
Infection with SARS-CoV-2 is associated with thromboinflammation, involving thrombotic and inflammatory responses, in many COVID-19 patients. In addition, immune dysfunction occurs in patients characterised by T cell exhaustion and severe lymphopenia. We investigated the distribution of phosphatidylserine (PS), a marker of dying cells, activated platelets and platelet-derived microparticles (PMP), during the clinical course of COVID-19. We found an unexpectedly high amount of blood cells loaded with PS+ PMPs for weeks after the initial COVID-19 diagnosis. Elevated frequencies of PS+ PMP+ PBMCs correlated strongly with increasing disease severity. As a marker, PS outperformed established laboratory markers for inflammation, leucocyte composition and coagulation, currently used for COVID-19 clinical scoring. PS+ PMPs preferentially bound to CD8+ T cells with gene expression signatures of proliferating effector rather than memory T cells. As PS+ PMPs carried programmed death-ligand 1 (PD-L1), they may affect T cell expansion or function. Our data provide a novel marker for disease severity and show that PS, which can trigger the blood coagulation cascade, the complement system, and inflammation, resides on activated immune cells. Therefore, PS may serve as a beacon to attract thromboinflammatory processes towards lymphocytes and cause immune dysfunction in COVID-19.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Phosphatidylserines / Leukocytes, Mononuclear / COVID-19 Limits: Adult / Humans Language: English Journal: J Extracell Vesicles Year: 2021 Document Type: Article Affiliation country: Jev2.12173

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Phosphatidylserines / Leukocytes, Mononuclear / COVID-19 Limits: Adult / Humans Language: English Journal: J Extracell Vesicles Year: 2021 Document Type: Article Affiliation country: Jev2.12173