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Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment.
Ramanathan, Santheraleka; Gopinath, Subash C B; Ismail, Zool Hilmi; Md Arshad, M K; Poopalan, Prabakaran.
  • Ramanathan S; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia.
  • Gopinath SCB; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia; Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia. Electronic address: subash@unimap.edu.my.
  • Ismail ZH; Centre for Artificial Intelligence and Robotics, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, 54100, Kuala Lumpur, Malaysia. Electronic address: zool@utm.my.
  • Md Arshad MK; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia; Faculty of Electronic Engineering Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Pauh Putra, Perlis, Malaysia.
  • Poopalan P; Faculty of Electronic Engineering Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Pauh Putra, Perlis, Malaysia.
Biosens Bioelectron ; 197: 113735, 2022 Feb 01.
Article in English | MEDLINE | ID: covidwho-1544823
ABSTRACT
In an aim of developing portable biosensor for SARS-CoV-2 pandemic, which facilitates the point-of-care aptasensing, a strategy using 10 µm gap-sized gold interdigitated electrode (AuIDE) is presented. The silane-modified AuIDE surface was deposited with ∼20 nm diamond and enhanced the detection of SARS-CoV-2 nucleocapsid protein (NCP). The characteristics of chemically modified diamond were evidenced by structural analyses, revealing the cubic crystalline nature at (220) and (111) planes as observed by XRD. XPS analysis denotes a strong interaction of carbon element, composed ∼95% as seen in EDS analysis. The C-C, CC, CO, CN functional groups were well-refuted from XPS spectra of carbon and oxygen elements in diamond. The interrelation between elements through FTIR analysis indicates major intrinsic bondings at 2687-2031 cm-1. The aptasensing was evaluated through electrochemical impedance spectroscopy measurements, using NCP spiked human serum. With a good selectivity the lower detection limit was evidenced as 0.389 fM, at a linear detection range from 1 fM to 100 pM. The stability, and reusability of the aptasensor were demonstrated, showing ∼30% and ∼33% loss of active state, respectively, after ∼11 days. The detection of NCP was evaluated by comparing anti-NCP aptamer and antibody as the bioprobes. The determination coefficients of R2 = 0.9759 and R2 = 0.9772 were obtained for aptamer- and antibody-based sensing, respectively. Moreover, the genuine interaction of NCP aptamer and protein was validated by enzyme linked apta-sorbent assay. The aptasensing strategy proposed with AuIDE/diamond enhanced sensing platform is highly recommended for early diagnosis of SARS-CoV-2 infection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Communicable Diseases / Aptamers, Nucleotide / Nanodiamonds / COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2021.113735

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Communicable Diseases / Aptamers, Nucleotide / Nanodiamonds / COVID-19 Type of study: Experimental Studies / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2022 Document Type: Article Affiliation country: J.bios.2021.113735