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Rapid Identification of SARS-CoV-2 Variants of Concern Using a Portable peakPCR Platform.
Bechtold, Philippe; Wagner, Philipp; Hosch, Salome; Siegrist, Denise; Ruiz-Serrano, Amalia; Gregorini, Michele; Mpina, Maxmillian; Ondó, Florentino Abaga; Obama, Justino; Ayekaba, Mitoha Ondo'o; Engler, Olivier; Stark, Wendelin J; Daubenberger, Claudia A; Schindler, Tobias.
  • Bechtold P; Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
  • Wagner P; Diaxxo AG, 8093 Zuerich, Switzerland.
  • Hosch S; Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
  • Siegrist D; University of Basel, 4051 Basel, Switzerland.
  • Ruiz-Serrano A; Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
  • Gregorini M; University of Basel, 4051 Basel, Switzerland.
  • Mpina M; Spiez Laboratory, 3700 Spiez, Switzerland.
  • Ondó FA; Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
  • Obama J; Diaxxo AG, 8093 Zuerich, Switzerland.
  • Ayekaba MO; Institute for Chemical and Bioengineering, ETH Zurich, 8093 Zuerich, Switzerland.
  • Engler O; Diaxxo AG, 8093 Zuerich, Switzerland.
  • Stark WJ; Swiss Tropical and Public Health Institute, 4051 Basel, Switzerland.
  • Daubenberger CA; University of Basel, 4051 Basel, Switzerland.
  • Schindler T; Laboratorio de Investigaciones de Baney, P.O. Box 338, Baney, Equatorial Guinea.
Anal Chem ; 93(49): 16350-16359, 2021 12 14.
Article in English | MEDLINE | ID: covidwho-1545571
ABSTRACT
The need for tools that facilitate rapid detection and continuous monitoring of SARS-CoV-2 variants of concern (VOCs) is greater than ever, as these variants are more transmissible and therefore increase the pressure of COVID-19 on healthcare systems. To address this demand, we aimed at developing and evaluating a robust and fast diagnostic approach for the identification of SARS-CoV-2 VOC-associated spike gene mutations. Our diagnostic assays detect the E484K and N501Y single-nucleotide polymorphisms (SNPs) as well as a spike gene deletion (HV69/70) and can be run on standard laboratory equipment or on the portable rapid diagnostic technology platform peakPCR. The assays achieved excellent diagnostic performance when tested with RNA extracted from culture-derived SARS-CoV-2 VOC lineages and clinical samples collected in Equatorial Guinea, Central-West Africa. Simplicity of usage and the relatively low cost are advantages that make our approach well suitable for decentralized and rapid testing, especially in resource-limited settings.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Variants Limits: Humans Country/Region as subject: Africa Language: English Journal: Anal Chem Year: 2021 Document Type: Article Affiliation country: Acs.analchem.1c02368

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Observational study / Prognostic study Topics: Variants Limits: Humans Country/Region as subject: Africa Language: English Journal: Anal Chem Year: 2021 Document Type: Article Affiliation country: Acs.analchem.1c02368