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The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous Expression.
Maffei, Mariano; Montemiglio, Linda Celeste; Vitagliano, Grazia; Fedele, Luigi; Sellathurai, Shaila; Bucci, Federica; Compagnone, Mirco; Chiarini, Valerio; Exertier, Cécile; Muzi, Alessia; Roscilli, Giuseppe; Vallone, Beatrice; Marra, Emanuele.
  • Maffei M; Evvivax Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Montemiglio LC; Institute of Molecular Biology and Pathology (IBPM), National Research Council, c/o Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza, University of Rome, P. le Aldo Moro, 5, 00185 Rome, Italy.
  • Vitagliano G; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Fedele L; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Sellathurai S; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Bucci F; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Compagnone M; Neomatrix Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Chiarini V; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Exertier C; Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza, University of Rome, P. le Aldo Moro, 5, 00185 Rome, Italy.
  • Muzi A; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Roscilli G; Evvivax Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Vallone B; Takis Biotech, Via di Castel Romano 100, 00128 Rome, Italy.
  • Marra E; Department of Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza, University of Rome, P. le Aldo Moro, 5, 00185 Rome, Italy.
Biomolecules ; 11(12)2021 12 02.
Article in English | MEDLINE | ID: covidwho-1551563
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
COVID-19 is a highly infectious disease caused by a newly emerged coronavirus (SARS-CoV-2) that has rapidly progressed into a pandemic. This unprecedent emergency has stressed the significance of developing effective therapeutics to fight the current and future outbreaks. The receptor-binding domain (RBD) of the SARS-CoV-2 surface Spike protein is the main target for vaccines and represents a helpful "tool" to produce neutralizing antibodies or diagnostic kits. In this work, we provide a detailed characterization of the native RBD produced in three major model systems Escherichia coli, insect and HEK-293 cells. Circular dichroism, gel filtration chromatography and thermal denaturation experiments indicated that recombinant SARS-CoV-2 RBD proteins are stable and correctly folded. In addition, their functionality and receptor-binding ability were further evaluated through ELISA, flow cytometry assays and bio-layer interferometry.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Year: 2021 Document Type: Article Affiliation country: Biom11121812

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Year: 2021 Document Type: Article Affiliation country: Biom11121812