Validation and Application of a Benchtop Cell Sorter in a Biosafety Level 3 Containment Setting.
Appl Biosaf
; 26(4): 205-209, 2021 Dec 01.
Article
in English
| MEDLINE | ID: covidwho-1554776
ABSTRACT
Introduction:
Fluorescent-activated cell sorting (FACS) is often the most appropriate technique to obtain pure populations of a cell type of interest for downstream analysis. However, aerosol droplets can be generated during the sort, which poses a biosafety risk when working with samples containing risk group 3 pathogens such as Francisella tularensis, Mycobacterium tuberculosis, Yersinia pestis, and severe acute respiratory syndrome coronavirus 2. For many researchers, placing the equipment required for FACS at biosafety level 3 (BSL-3) is often not possible due to expense, space, or expertise available.Methods:
We performed aerosol testing as part of the biosafety evaluation of the MACSQuant Tyto, a completely closed, cartridge-based cell sorter. We also established quality control procedures to routinely evaluate instrument performance.Results:
The MACSQuant Tyto does not produce aerosols as part of the sort procedure.Discussion:
These data serve as guidance for other facilities with containment laboratories wishing to use the MACSQuant Tyto for cell sorting. Potential users should consult with their Institutional Biosafety Committees to perform in-house risk assessments of this equipment.Conclusion:
The MACSQuant Tyto can safely be used on the benchtop to sort samples at BSL-3.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Type of study:
Experimental Studies
/
Prognostic study
Language:
English
Journal:
Appl Biosaf
Year:
2021
Document Type:
Article
Affiliation country:
APB.20.0065
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