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Fast, Reliable, and Simple Point-of-Care-like Adaptation of RT-qPCR for the Detection of SARS-CoV-2 for Use in Hospital Emergency Departments.
Pavletic, Martina; Mazor, Marija; Lerga, Mate; Mileta, Tatjana; Zeleznjak, Jelena; Ruzic, Tina; Ravlic, Sanda; Palcevski, Dora; Kirincic, Jelena; Meznaric, Silvestar; Miskovic, Ana; Materljan, Maja; Sustic, Alan; Lisnic, Berislav; Juranic Lisnic, Vanda.
  • Pavletic M; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Mazor M; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  • Lerga M; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  • Mileta T; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Zeleznjak J; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  • Ruzic T; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Ravlic S; Department of Anesthesiology, Reanimatology, Intensive Care and Emergency Medicine, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  • Palcevski D; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  • Kirincic J; Center for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  • Meznaric S; Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, 10000 Zagreb, Croatia.
  • Miskovic A; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Materljan M; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Sustic A; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Lisnic B; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
  • Juranic Lisnic V; Emergency Department, Clinical Hospital Center Rijeka, 51000 Rijeka, Croatia.
Viruses ; 13(12)2021 12 02.
Article in English | MEDLINE | ID: covidwho-1554951
ABSTRACT
During COVID-19 pandemics, the availability of testing has often been a limiting factor during patient admissions into the hospital. To circumvent this problem, we adapted an existing diagnostic assay, Seegene Allplex SARS-CoV-2, into a point-of-care-style direct qPCR (POC dqPCR) assay and implemented it in the Emergency Department of Clinical Hospital Center Rijeka, Croatia. In a 4-month analysis, we tested over 10,000 patients and demonstrated that POC-dqPCR is robust and reliable and can be successfully implemented in emergency departments and similar near-patient settings and can be performed by medical personnel with little prior experience in qPCR.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Emergency Service, Hospital / Point-of-Care Testing / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Humans Country/Region as subject: Europa Language: English Year: 2021 Document Type: Article Affiliation country: V13122413

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Emergency Service, Hospital / Point-of-Care Testing / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Prognostic study Limits: Humans Country/Region as subject: Europa Language: English Year: 2021 Document Type: Article Affiliation country: V13122413