Your browser doesn't support javascript.
Porcine Deltacoronavirus Utilizes Sialic Acid as an Attachment Receptor and Trypsin Can Influence the Binding Activity.
Yuan, Yixin; Zu, Shaopo; Zhang, Yunfei; Zhao, Fujie; Jin, Xiaohui; Hu, Hui.
  • Yuan Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Zu S; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Zhang Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Zhao F; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Jin X; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
  • Hu H; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
Viruses ; 13(12)2021 12 06.
Article in English | MEDLINE | ID: covidwho-1555020
ABSTRACT
Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in nursing piglets. Studies showed that PDCoV uses porcine aminopeptidase N (pAPN) as an entry receptor, but the infection of pAPN-knockout cells or pigs with PDCoV revealed that pAPN might be not a critical functional receptor, implying there exists an unidentified receptor involved in PDCoV infection. Herein, we report that sialic acid (SA) can act as an attachment receptor for PDCoV invasion and facilitate its infection. We first demonstrated that the carbohydrates destroyed on the cell membrane using NaIO4 can alleviate the susceptibility of cells to PDCoV. Further study showed that the removal of SA, a typical cell-surface carbohydrate, could influence the PDCoV infectivity to the cells significantly, suggesting that SA was involved in the infection. The results of plaque assay and Western blotting revealed that SA promoted PDCoV infection by increasing the number of viruses binding to SA on the cell surface during the adsorption phase, which was also confirmed by atomic force microscopy at the microscopic level. In in vivo experiments, we found that the distribution levels of PDCoV and SA were closely relevant in the swine intestine, which contains huge amount of trypsin. We further confirmed that SA-binding capacity to PDCoV is related to the pre-treatment of PDCoV with trypsin. In conclusion, SA is a novel attachment receptor for PDCoV infection to enhance its attachment to cells, which is dependent on the pre-treatment of trypsin on PDCoV. This study paves the way for dissecting the mechanisms of PDCoV-host interactions and provides new strategies to control PDCoV infection.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Receptors, Virus / Trypsin / N-Acetylneuraminic Acid / Virus Attachment / Deltacoronavirus Limits: Animals Language: English Year: 2021 Document Type: Article Affiliation country: V13122442

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Receptors, Virus / Trypsin / N-Acetylneuraminic Acid / Virus Attachment / Deltacoronavirus Limits: Animals Language: English Year: 2021 Document Type: Article Affiliation country: V13122442