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Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.
Melani, Rafael D; Des Soye, Benjamin J; Kafader, Jared O; Forte, Eleonora; Hollas, Michael; Blagojevic, Voislav; Negrão, Fernanda; McGee, John P; Drown, Bryon; Lloyd-Jones, Cameron; Seckler, Henrique S; Camarillo, Jeannie M; Compton, Philip D; LeDuc, Richard D; Early, Bryan; Fellers, Ryan T; Cho, Byoung-Kyu; Mattamana, Basil Baby; Goo, Young Ah; Thomas, Paul M; Ash, Michelle K; Bhimalli, Pavan P; Al-Harthi, Lena; Sha, Beverly E; Schneider, Jeffrey R; Kelleher, Neil L.
  • Melani RD; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Des Soye BJ; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Kafader JO; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.
  • Forte E; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Hollas M; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.
  • Blagojevic V; Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, United States.
  • Negrão F; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • McGee JP; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Drown B; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Lloyd-Jones C; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Seckler HS; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Camarillo JM; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Compton PD; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • LeDuc RD; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Early B; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Fellers RT; Integrated Protein Technologies, Evanston, Illinois 60201, United States.
  • Cho BK; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Mattamana BB; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Goo YA; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Thomas PM; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.
  • Ash MK; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.
  • Bhimalli PP; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Al-Harthi L; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.
  • Sha BE; Departments of Molecular Biosciences, Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
  • Schneider JR; Proteomics Center of Excellence, Evanston, Illinois 60208, United States.
  • Kelleher NL; Department of Microbial Pathogens and Immunity, Rush University Medical Center, Chicago, Illinois 60612, United States.
J Proteome Res ; 21(1): 274-288, 2022 01 07.
Article in English | MEDLINE | ID: covidwho-1560547
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multiparametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We applied Ig-MS to plasma from subjects with severe and mild COVID-19 and immunized subjects after two vaccine doses, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, that could use other antigens of interest to gauge immune responses to vaccination, pathogens, or autoimmune disorders.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: J Proteome Res Journal subject: Biochemistry Year: 2022 Document Type: Article Affiliation country: Acs.jproteome.1c00882

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Topics: Vaccines Limits: Humans Language: English Journal: J Proteome Res Journal subject: Biochemistry Year: 2022 Document Type: Article Affiliation country: Acs.jproteome.1c00882