Your browser doesn't support javascript.
In vitro and clinical studies on the efficacy of α-cyclodextrin and hydroxytyrosol against SARS-CoV-2 infection.
Eur Rev Med Pharmacol Sci ; 25(1 Suppl): 81-89, 2021 12.
Article in English | MEDLINE | ID: covidwho-1566966
ABSTRACT

OBJECTIVE:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a new coronavirus responsible for the current pandemic of coronavirus disease 2019 (COVID-19). This virus attacks cells of the airway epithelium by binding transmembrane angiotensin-converting enzyme 2 (ACE2). Hydroxytyrosol has anti-viral properties. Alpha-cyclodextrin can deplete sphingolipids and phospholipids from cell membranes. The aim of the present experimental study was to evaluate the efficacy of α-cyclodextrin and hydroxytyrosol in improving defenses against SARS-CoV-2 infection in in vitro cell models and humans. PATIENTS AND

METHODS:

For in vitro experiments on Vero E6 cells, RNA for RT-qPCR analysis was extracted from Caco2 and human fibroblast cell lines. For study in humans, the treatment group consisted of 149 healthy volunteers in Northern Cyprus, considered at higher risk of SARS-CoV-2 infection than the general population. The volunteers used nasal spray containing α-cyclodextrin and hydroxytyrosol for 4 weeks. The control group consisted of 76 healthy volunteers who did not use the spray.

RESULTS:

RT-qPCR experiments on targeted genes involved in endocytosis showed a reduction in gene expression, whereas cytotoxicity and cytoprotective tests showed that the compounds exerted a protective effect against SARS-CoV-2 infection at non-cytotoxic concentrations. None of the volunteers became positive to SARS-CoV-2 RT-qPCR assay during the 30 days of treatment.

CONCLUSIONS:

Treatment with α-cyclodextrin and hydroxytyrosol nasal spray improved defenses against SARS-CoV-2 infection and reduced synthesis of viral particles.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Phenylethyl Alcohol / Alpha-Cyclodextrins / Virus Internalization / SARS-CoV-2 / Anti-Infective Agents Type of study: Experimental Studies / Prognostic study Limits: Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Eur Rev Med Pharmacol Sci Journal subject: Pharmacology / Toxicology Year: 2021 Document Type: Article Affiliation country: Eurrev_202112_27337

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Phenylethyl Alcohol / Alpha-Cyclodextrins / Virus Internalization / SARS-CoV-2 / Anti-Infective Agents Type of study: Experimental Studies / Prognostic study Limits: Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Eur Rev Med Pharmacol Sci Journal subject: Pharmacology / Toxicology Year: 2021 Document Type: Article Affiliation country: Eurrev_202112_27337