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Recovery of SARS-CoV-2 from large volumes of raw wastewater is enhanced with the inuvai R180 system.
Monteiro, Silvia; Rente, Daniela; Cunha, Mónica V; Marques, Tiago A; Cardoso, Eugénia; Álvaro, Pedro; Vilaça, João; Ribeiro, Jorge; Silva, Marco; Coelho, Norberta; Brôco, Nuno; Carvalho, Marta; Santos, Ricardo.
  • Monteiro S; Laboratorio de Análises, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal. Electronic address: silvia.monteiro@tecnico.ulisboa.pt.
  • Rente D; Laboratorio de Análises, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
  • Cunha MV; Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências, Universidade de Lisboa, 1749-016, Lisboa, Portugal; Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, 1749-016, Lisboa, Portugal.
  • Marques TA; Centre for Research Into Ecological and Environmental Modelling, The Observatory, University of St Andrews, St Andrews, KY16 9LZ, Scotland, UK; Centro de Estatística e Aplicações, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749-016, Lisboa, Portugal.
  • Cardoso E; Águas Do Tejo Atlântico, Fábrica de Águas de Alcântara, Avenida de Ceuta, 1300-254, Lisboa, Portugal.
  • Álvaro P; Águas Do Tejo Atlântico, Fábrica de Águas de Alcântara, Avenida de Ceuta, 1300-254, Lisboa, Portugal.
  • Vilaça J; SIMDOURO, ETAR de Gaia Litoral, 4400-356, Canidelo, Portugal.
  • Ribeiro J; SIMDOURO, ETAR de Gaia Litoral, 4400-356, Canidelo, Portugal.
  • Silva M; Águas Do Norte, Lugar de Gaído, 4755-045, Barcelos, Portugal.
  • Coelho N; Águas Do Norte, Lugar de Gaído, 4755-045, Barcelos, Portugal.
  • Brôco N; AdP VALOR, Serviços Ambientais, S.A., Rua Visconde de Seabra, 3, 1700-421, Lisboa, Portugal.
  • Carvalho M; AdP VALOR, Serviços Ambientais, S.A., Rua Visconde de Seabra, 3, 1700-421, Lisboa, Portugal.
  • Santos R; Laboratorio de Análises, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.
J Environ Manage ; 304: 114296, 2022 Feb 15.
Article in English | MEDLINE | ID: covidwho-1568834
Preprint
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ABSTRACT
Wastewater-based epidemiology (WBE) for severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is a powerful tool to complement syndromic surveillance. Although detection of SARS-CoV-2 in raw wastewater may be prompted with good recoveries during periods of high community prevalence, in the early stages of population outbreaks concentration procedures are required to overcome low viral concentrations. Several methods have become available for the recovery of SARS-CoV-2 from raw wastewater, generally involving filtration. However, these methods are limited to small sample volumes, possibly missing the early stages of virus circulation, and restrained applicability across different water matrices. The aim of this study was thus to evaluate the performance of three methods enabling the concentration of SARS-CoV-2 from large volumes of wastewater i) hollow fiber filtration using the inuvai R180, with an enhanced elution protocol and polyethylene glycol (PEG) precipitation; ii) PEG precipitation; and iii) skimmed milk flocculation. The performance of the three approaches was evaluated in wastewater from multiple wastewater treatment plants (WWTP) with distinct singularities, according to i) effective volume; ii) percentage of recovery; iii) extraction efficiency; iv) inhibitory effect; and v) the limits of detection and quantification. The inuvai R180 system had the best performance, with detection of spiked control across all samples, with average recovery percentages of 68% for porcine epidemic diarrhea virus (PEDV), with low variability. Mean recoveries for PEG precipitation and skimmed milk flocculation were 9% and 14%, respectively. The inuvai R180 enables the scalability of volumes without negative impact on the costs, time for analysis, and recovery/inhibition. Moreover, hollow fiber ultrafilters favor the concentration of different microbial taxonomic groups. Such combined features make this technology attractive for usage in environmental waters monitoring.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viruses / COVID-19 Type of study: Experimental Studies / Observational study / Randomized controlled trials Limits: Animals / Humans Language: English Journal: J Environ Manage Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viruses / COVID-19 Type of study: Experimental Studies / Observational study / Randomized controlled trials Limits: Animals / Humans Language: English Journal: J Environ Manage Year: 2022 Document Type: Article