High-quality RNA improves sensitivity of SARS-CoV-2 detection by colorimetric RT-LAMP.
Exp Biol Med (Maywood)
; 247(3): 276-281, 2022 02.
Article
in English
| MEDLINE | ID: covidwho-1571696
ABSTRACT
The global SARS-CoV-2 pandemic requires a rapid, reliable, and user-friendly diagnostic test to help control the spread of the virus. Reverse transcription and quantitative PCR (RT-qPCR) is currently the gold standard method for SARS-CoV-2 detection. Here, we develop a protocol based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and demonstrate increased sensitivity of this technique using fresh RNA extracts compared to RNA samples subjected to freezing/thawing cycles. We further compare RT-LAMP to RT-qPCR and demonstrate that the RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct values between 8 and 35.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
RNA, Viral
/
Nucleic Acid Amplification Techniques
/
Molecular Diagnostic Techniques
/
COVID-19 Nucleic Acid Testing
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Exp Biol Med (Maywood)
Journal subject:
Biology
/
Physiology
/
Medicine
Year:
2022
Document Type:
Article
Affiliation country:
15353702211054768
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