InterARTIC: an interactive web application for whole-genome nanopore sequencing analysis of SARS-CoV-2 and other viruses.
Bioinformatics
; 2021 Dec 15.
Article
in English
| MEDLINE | ID: covidwho-1574291
ABSTRACT
MOTIVATION InterARTIC is an interactive web application for the analysis of viral whole-genome sequencing (WGS) data generated on Oxford Nanopore Technologies (ONT) devices. A graphical interface enables users with no bioinformatics expertise to analyse WGS experiments and reconstruct consensus genome sequences from individual isolates of viruses, such as SARS-CoV-2. InterARTIC is intended to facilitate widespread adoption and standardisation of ONT sequencing for viral surveillance and molecular epidemiology. WORKED EXAMPLE We demonstrate the use of InterARTIC for the analysis of ONT viral WGS data from SARS-CoV-2 and Ebola virus, using a laptop computer or the internal computer on an ONT GridION sequencing device. We showcase the intuitive graphical interface, workflow customisation capabilities and job-scheduling system that facilitate execution of small- and large-scale WGS projects on any common virus. IMPLEMENTATION InterARTIC is a free, open-source web application implemented in Python that executes best-practice command line workflows from the ARTIC network. The application can be downloaded as a set of pre-compiled binaries that are compatible with all common Linux distributions, Windows with Linux subsystems, MacOSX and ARM systems. For further details please visit https//github.com/Psy-Fer/interARTIC/. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Language:
English
Journal subject:
Medical Informatics
Year:
2021
Document Type:
Article
Affiliation country:
Bioinformatics
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