Automated Reverse Transcription Polymerase Chain Reaction Data Analysis for Sars-CoV-2 Detection.
Rev Invest Clin
; 73(6): 339-346, 2021 11 05.
Article
in English
| MEDLINE | ID: covidwho-1574413
ABSTRACT
BACKGROUND:
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is a current public health concern. Rapid diagnosis is crucial, and reverse transcription polymerase chain reaction (RT-PCR) is presently the reference standard for SARS-CoV-2 detection.OBJECTIVE:
Automated RT-PCR analysis (ARPA) is a software designed to analyze RT-PCR data for SARSCoV-2 detection. ARPA loads the RT-PCR data, classifies each sample by assessing its amplification curve behavior, evaluates the experiment's quality, and generates reports.METHODS:
ARPA was implemented in the R language and deployed as a Shiny application. We evaluated the performance of ARPA in 140 samples. The samples were manually classified and automatically analyzed using ARPA.RESULTS:
ARPA had a true-positive rate = 1, true-negative rate = 0.98, positive-predictive value = 0.95, and negative-predictive value = 1, with 36 samples correctly classified as positive, 100 samples correctly classified as negative, and two samples classified as positive even when labeled as negative by manual inspection. Two samples were labeled as invalid by ARPA and were not considered in the performance metrics calculation.CONCLUSIONS:
ARPA is a sensitive and specific software that facilitates the analysis of RT-PCR data, and its implementation can reduce the time required in the diagnostic pipeline.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Software
/
Diagnosis, Computer-Assisted
/
SARS-CoV-2
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Rev Invest Clin
Journal subject:
Medicine
Year:
2021
Document Type:
Article
Affiliation country:
RIC.21000189
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