Your browser doesn't support javascript.
Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS).
Sharma, Nupur; Bhat, Sadam H; Tripathi, Gaurav; Yadav, Manisha; Mathew, Babu; Bindal, Vasundhra; Sharma, Shvetank; Gupta, Ekta; Maras, Jaswinder Singh; Sarin, Shiv Kumar.
  • Sharma N; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Bhat SH; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Tripathi G; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Yadav M; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Mathew B; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Bindal V; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Sharma S; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Gupta E; Departments of Virology, Institute of Liver and Biliary Sciences, New Delhi, India.
  • Maras JS; Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
  • Sarin SK; Department of Hepatology, Institute of Liver and Biliary Sciences, New Delhi 110070, India.
STAR Protoc ; 3(1): 101051, 2022 03 18.
Article in English | MEDLINE | ID: covidwho-1575581
ABSTRACT
Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).
Subject(s)
Keywords
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Chromatography, High Pressure Liquid / Metabolomics / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2021.101051

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: Available Collection: International databases Database: MEDLINE Main subject: Chromatography, High Pressure Liquid / Metabolomics / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: STAR Protoc Year: 2022 Document Type: Article Affiliation country: J.xpro.2021.101051