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A virus-derived microRNA targets immune response genes during SARS-CoV-2 infection.
Singh, Meetali; Chazal, Maxime; Quarato, Piergiuseppe; Bourdon, Loan; Malabat, Christophe; Vallet, Thomas; Vignuzzi, Marco; van der Werf, Sylvie; Behillil, Sylvie; Donati, Flora; Sauvonnet, Nathalie; Nigro, Giulia; Bourgine, Maryline; Jouvenet, Nolwenn; Cecere, Germano.
  • Singh M; Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Mechanisms of Epigenetic Inheritance, Université de Paris, Paris, France.
  • Chazal M; Virus Sensing and Signaling Unit, Institut Pasteur, CNRS UMR3569, Université de Paris, Paris, France.
  • Quarato P; Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Mechanisms of Epigenetic Inheritance, Université de Paris, Paris, France.
  • Bourdon L; Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Mechanisms of Epigenetic Inheritance, Université de Paris, Paris, France.
  • Malabat C; Department of Computational Biology, Institut Pasteur, Bioinformatics and Biostatistics Hub, Université de Paris, Paris, France.
  • Vallet T; Viral Populations and Pathogenesis Unit, Institut Pasteur, CNRS UMR 3569, Université de Paris, Paris, France.
  • Vignuzzi M; Viral Populations and Pathogenesis Unit, Institut Pasteur, CNRS UMR 3569, Université de Paris, Paris, France.
  • van der Werf S; National Reference Center for Respiratory Viruses, Molecular Genetics of RNA Viruses, Institut Pasteur, CNRS UMR 3569, Université de Paris, Paris, France.
  • Behillil S; National Reference Center for Respiratory Viruses, Molecular Genetics of RNA Viruses, Institut Pasteur, CNRS UMR 3569, Université de Paris, Paris, France.
  • Donati F; National Reference Center for Respiratory Viruses, Molecular Genetics of RNA Viruses, Institut Pasteur, CNRS UMR 3569, Université de Paris, Paris, France.
  • Sauvonnet N; Intracellular Trafficking and Tissue Homeostasis, Institut Pasteur, Université de Paris, Paris, France.
  • Nigro G; Microenvironment and Immunity Unit, Institut Pasteur, INSERM U1224, Université de Paris, Paris, France.
  • Bourgine M; Virology Department, Institut Pasteur, Institut Pasteur-TheraVectys Joint Lab, Université de Paris, Paris, France.
  • Jouvenet N; Virus Sensing and Signaling Unit, Institut Pasteur, CNRS UMR3569, Université de Paris, Paris, France.
  • Cecere G; Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Mechanisms of Epigenetic Inheritance, Université de Paris, Paris, France.
EMBO Rep ; 23(2): e54341, 2022 02 03.
Article in English | MEDLINE | ID: covidwho-1575628
ABSTRACT
SARS-CoV-2 infection results in impaired interferon response in patients with severe COVID-19. However, how SARS-CoV-2 interferes with host immune responses is incompletely understood. Here, we sequence small RNAs from SARS-CoV-2-infected human cells and identify a microRNA (miRNA) derived from a recently evolved region of the viral genome. We show that the virus-derived miRNA produces two miRNA isoforms in infected cells by the enzyme Dicer, which are loaded into Argonaute proteins. Moreover, the predominant miRNA isoform targets the 3'UTR of interferon-stimulated genes and represses their expression in a miRNA-like fashion. Finally, the two viral miRNA isoforms were detected in nasopharyngeal swabs from COVID-19 patients. We propose that SARS-CoV-2 can potentially employ a virus-derived miRNA to hijack the host miRNA machinery, which could help to evade the interferon-mediated immune response.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / MicroRNAs / SARS-CoV-2 / COVID-19 Limits: Humans Language: English Journal: EMBO Rep Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: Embr.202154341

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / MicroRNAs / SARS-CoV-2 / COVID-19 Limits: Humans Language: English Journal: EMBO Rep Journal subject: Molecular Biology Year: 2022 Document Type: Article Affiliation country: Embr.202154341