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Antiviral Activities of Carbazole Derivatives against Porcine Epidemic Diarrhea Virus In Vitro.
Chen, Zheng; Chen, Jinfeng; Wei, Xiaodong; Hua, Huiying; Hu, Ruiming; Ding, Nengshui; Zhang, Jinhua; Song, Deping; Ye, Yu; Tang, Yuxin; Ding, Zhen; Ke, Shaoyong.
  • Chen Z; Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Chen J; Jiangxi Engineering Research Center for Animal Health Products, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Wei X; Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Hua H; Jiangxi Engineering Research Center for Animal Health Products, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Hu R; Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Ding N; Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Zhang J; Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Song D; State Key Laboratory for Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Ye Y; Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Tang Y; Jiangxi Engineering Research Center for Animal Health Products, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Ding Z; Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
  • Ke S; Jiangxi Engineering Research Center for Animal Health Products, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Viruses ; 13(12)2021 12 16.
Article in English | MEDLINE | ID: covidwho-1576965
ABSTRACT
Porcine epidemic diarrhea virus (PEDV), an enteric coronavirus, causes neonatal pig acute gastrointestinal infection with a characterization of severe diarrhea, vomiting, high morbidity, and high mortality, resulting in tremendous damages to the swine industry. Neither specific antiviral drugs nor effective vaccines are available, posing a high priority to screen antiviral drugs. The aim of this study is to investigate anti-PEDV effects of carbazole alkaloid derivatives. Eighteen carbazole derivatives (No.1 to No.18) were synthesized, and No.5, No.7, and No.18 were identified to markedly reduce the replication of enhanced green fluorescent protein (EGFP) inserted-PEDV, and the mRNA level of PEDV N. Flow cytometry assay, coupled with CCK8 assay, confirmed No.7 and No.18 carbazole derivatives displayed high inhibition effects with low cell toxicity. Furthermore, time course analysis indicated No.7 and No.18 carbazole derivatives exerted inhibition at the early stage of the viral life cycle. Collectively, the analysis underlines the benefit of carbazole derivatives as potential inhibitors of PEDV, and provides candidates for the development of novel therapeutic agents.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Carbazoles / Porcine epidemic diarrhea virus Type of study: Prognostic study Topics: Vaccines Limits: Animals Language: English Year: 2021 Document Type: Article Affiliation country: V13122527

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Carbazoles / Porcine epidemic diarrhea virus Type of study: Prognostic study Topics: Vaccines Limits: Animals Language: English Year: 2021 Document Type: Article Affiliation country: V13122527