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Minimally Invasive Tissue Sampling Findings in 12 Patients With Coronavirus Disease 2019.
Rakislova, Natalia; Rodrigo-Calvo, Maria Teresa; Marimon, Lorena; Ribera-Cortada, Inmaculada; Ismail, Mamudo R; Carrilho, Carla; Fernandes, Fabiola; Ferrando, Melania; Sanfeliu, Esther; Castillo, Paola; Guerrero, José; Ramírez-Ruz, José; Saez de Gordoa, Karmele; López Del Campo, Ricardo; Bishop, Rosanna; Ortiz, Estrella; Muñoz-Beatove, Abel; Vila, Jordi; Hurtado, Juan Carlos; Navarro, Mireia; Maixenchs, Maria; Delgado, Vima; Aldecoa, Iban; Martinez-Pozo, Antonio; Castro, Pedro; Menéndez, Clara; Bassat, Quique; Martinez, Miguel J; Ordi, Jaume.
  • Rakislova N; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Rodrigo-Calvo MT; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Marimon L; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Ribera-Cortada I; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Ismail MR; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Carrilho C; Department of Pathology, Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique.
  • Fernandes F; Department of Pathology, Maputo Central Hospital, Maputo, Mozambique.
  • Ferrando M; Department of Pathology, Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique.
  • Sanfeliu E; Department of Pathology, Maputo Central Hospital, Maputo, Mozambique.
  • Castillo P; Department of Pathology, Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique.
  • Guerrero J; Department of Pathology, Maputo Central Hospital, Maputo, Mozambique.
  • Ramírez-Ruz J; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Saez de Gordoa K; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • López Del Campo R; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Bishop R; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Ortiz E; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Muñoz-Beatove A; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Vila J; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Hurtado JC; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Navarro M; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Maixenchs M; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Delgado V; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Aldecoa I; Department of Microbiology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Martinez-Pozo A; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Castro P; Department of Microbiology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Menéndez C; Department of Microbiology, Hospital Clínic, Universitat de Barcelona, Spain.
  • Bassat Q; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Martinez MJ; ISGlobal, Barcelona Institute for Global Health, Hospital Clínic-Universitat de Barcelona, Spain.
  • Ordi J; Department of Pathology, Hospital Clínic, Universitat de Barcelona, Spain.
Clin Infect Dis ; 73(Suppl_5): S454-S464, 2021 12 15.
Article in English | MEDLINE | ID: covidwho-1577471
ABSTRACT

BACKGROUND:

Minimally invasive tissue sampling (MITS), a postmortem procedure that uses core needle biopsy samples and does not require opening the body, may be a valid alternative to complete autopsy (CA) in highly infectious diseases such as coronavirus disease-19 (COVID-19). This study aimed to (1) compare the performance of MITS and CA in a series of COVID-19 deaths and (2) evaluate the safety of the procedure.

METHODS:

From October 2020 to February 2021, MITS was conducted in 12 adults who tested positive before death for COVID-19, in a standard, well-ventilated autopsy room, where personnel used reinforced personal protective equipment. In 9 cases, a CA was performed after MITS. A thorough histological evaluation was conducted, and the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was evaluated by real-time reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry.

RESULTS:

The diagnoses provided by MITS and CA matched almost perfectly. In 9 patients, COVID-19 was in the chain of events leading to death, being responsible for diffuse alveolar damage and mononuclear T-cell inflammatory response in the lungs. No specific COVID-19 features were identified. Three deaths were not related to COVID-19. All personnel involved in MITS repeatedly tested negative for COVID-19. SARS-CoV-2 was identified by RT-PCR and immunohistochemistry in the MITS samples, particularly in the lungs.

CONCLUSIONS:

MITS is useful for evaluating COVID-19-related deaths in settings where a CA is not feasible. The results of this simplified and safer technique are comparable to those of CA.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Journal: Clin Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: CID

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Experimental Studies Limits: Humans Language: English Journal: Clin Infect Dis Journal subject: Communicable Diseases Year: 2021 Document Type: Article Affiliation country: CID