Your browser doesn't support javascript.
Detection of A-to-I RNA Editing in SARS-COV-2.
Picardi, Ernesto; Mansi, Luigi; Pesole, Graziano.
  • Picardi E; Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari "Aldo Moro", Via Orabona 4, 70125 Bari, Italy.
  • Mansi L; Institute of Biomembranes and Bioenergetics, National Research Council, Via Amendola 122/O, 70126 Bari, Italy.
  • Pesole G; Consorzio Interuniversitario Biotecnologie (CIB), 34012 Trieste, Italy.
Genes (Basel) ; 13(1)2021 12 23.
Article in English | MEDLINE | ID: covidwho-1580896
ABSTRACT
ADAR1-mediated deamination of adenosines in long double-stranded RNAs plays an important role in modulating the innate immune response. However, recent investigations based on metatranscriptomic samples of COVID-19 patients and SARS-COV-2-infected Vero cells have recovered contrasting findings. Using RNAseq data from time course experiments of infected human cell lines and transcriptome data from Vero cells and clinical samples, we prove that A-to-G changes observed in SARS-COV-2 genomes represent genuine RNA editing events, likely mediated by ADAR1. While the A-to-I editing rate is generally low, changes are distributed along the entire viral genome, are overrepresented in exonic regions, and are (in the majority of cases) nonsynonymous. The impact of RNA editing on virus-host interactions could be relevant to identify potential targets for therapeutic interventions.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Adenosine Deaminase / RNA-Binding Proteins / Genome, Viral / RNA Editing / Host-Pathogen Interactions / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Language: English Year: 2021 Document Type: Article Affiliation country: Genes13010041

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Adenosine Deaminase / RNA-Binding Proteins / Genome, Viral / RNA Editing / Host-Pathogen Interactions / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Language: English Year: 2021 Document Type: Article Affiliation country: Genes13010041