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Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection.
Peng, Tao; Jiao, Xueshima; Liang, Zhanwei; Zhao, Hongwei; Zhao, Yang; Xie, Jie; Jiang, You; Yu, Xiaoping; Fang, Xiang; Dai, Xinhua.
  • Peng T; Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
  • Jiao X; Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
  • Liang Z; College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
  • Zhao H; Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
  • Zhao Y; College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
  • Xie J; College of Ecology and Environment, Hainan University, Haikou 570228, China.
  • Jiang Y; Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
  • Yu X; Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
  • Fang X; Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China.
  • Dai X; College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Biosensors (Basel) ; 12(1)2021 Dec 29.
Article in English | MEDLINE | ID: covidwho-2276106
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 µg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoassay / Copper / Metal Nanoparticles / Coronavirus Nucleocapsid Proteins / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Bios12010013

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoassay / Copper / Metal Nanoparticles / Coronavirus Nucleocapsid Proteins / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Bios12010013