Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virus.
Eur J Med Res
; 26(1): 147, 2021 Dec 17.
Article
in English
| MEDLINE | ID: covidwho-1582004
ABSTRACT
BACKGROUND:
The outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.METHODS:
RT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.RESULTS:
The performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/µL and lower limit of quantification (LLOQ) as 10E2 copies/µL.CONCLUSION:
A quantitative detection of the COVID-19 virus based on RT-PCR was established.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Reverse Transcriptase Polymerase Chain Reaction
/
Coronavirus RNA-Dependent RNA Polymerase
/
Coronavirus Nucleocapsid Proteins
/
Coronavirus Envelope Proteins
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
Topics:
Vaccines
Limits:
Humans
Language:
English
Journal:
Eur J Med Res
Journal subject:
Medicine
Year:
2021
Document Type:
Article
Affiliation country:
S40001-021-00608-5
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