Paper-based microfluidic chip for rapid detection of SARS-CoV-2 N protein.
Bioengineered
; 13(1): 876-883, 2022 01.
Article
in English
| MEDLINE | ID: covidwho-1585254
ABSTRACT
This research has developed a method for rapid detection of SARS-CoV-2 N protein on a paper-based microfluidic chip. The chitosan-glutaraldehyde cross-linking method is used to fix the coated antibody, and the sandwich enzyme-linked immunosorbent method is used to achieve the specific detection of the target antigen. The system studied the influence of coating antibody concentration and enzyme-labeled antibody concentration on target antigen detection. According to the average gray value measured under different N protein concentrations, the standard curve of the method was established and the sensitivity was tested, and its linear regression was obtained. The equation is y = 9.8286x+137.6, R2 = 0.9772 > 0.90, which shows a high degree of fit. When the concentration of coating antibody and enzyme-labeled antibody were 1 µg/mL and 2 µg/mL, P > 0.05, the difference was not statistically significant, so the lower concentration of 1 µg/mL was chosen as the coating antibody concentration. The results show that the minimum concentration of N protein that can be detected by this method is 8 µg/mL, and the minimum concentration of coating antibody and enzyme-labeled antibody is 1 µg/mL, which has the characteristics of high sensitivity and good repeatability.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Lab-On-A-Chip Devices
/
Coronavirus Nucleocapsid Proteins
/
COVID-19 Serological Testing
/
SARS-CoV-2
/
Antigens, Viral
Type of study:
Diagnostic study
/
Prognostic study
/
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
Bioengineered
Year:
2022
Document Type:
Article
Affiliation country:
21655979.2021.2014385
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