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Paper-based microfluidic chip for rapid detection of SARS-CoV-2 N protein.
Sun, Mingdi; Han, Man; Xu, Shengnan; Yan, Kai; Nigal, Gul; Zhang, Tongyang; Song, Bo.
  • Sun M; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
  • Han M; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
  • Xu S; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
  • Yan K; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
  • Nigal G; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
  • Zhang T; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
  • Song B; Medical Technology College, Qiqihar Medical University, Qiqihar, China.
Bioengineered ; 13(1): 876-883, 2022 01.
Article in English | MEDLINE | ID: covidwho-1585254
ABSTRACT
This research has developed a method for rapid detection of SARS-CoV-2 N protein on a paper-based microfluidic chip. The chitosan-glutaraldehyde cross-linking method is used to fix the coated antibody, and the sandwich enzyme-linked immunosorbent method is used to achieve the specific detection of the target antigen. The system studied the influence of coating antibody concentration and enzyme-labeled antibody concentration on target antigen detection. According to the average gray value measured under different N protein concentrations, the standard curve of the method was established and the sensitivity was tested, and its linear regression was obtained. The equation is y = 9.8286x+137.6, R2 = 0.9772 > 0.90, which shows a high degree of fit. When the concentration of coating antibody and enzyme-labeled antibody were 1 µg/mL and 2 µg/mL, P > 0.05, the difference was not statistically significant, so the lower concentration of 1 µg/mL was chosen as the coating antibody concentration. The results show that the minimum concentration of N protein that can be detected by this method is 8 µg/mL, and the minimum concentration of coating antibody and enzyme-labeled antibody is 1 µg/mL, which has the characteristics of high sensitivity and good repeatability.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Lab-On-A-Chip Devices / Coronavirus Nucleocapsid Proteins / COVID-19 Serological Testing / SARS-CoV-2 / Antigens, Viral Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Bioengineered Year: 2022 Document Type: Article Affiliation country: 21655979.2021.2014385

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Lab-On-A-Chip Devices / Coronavirus Nucleocapsid Proteins / COVID-19 Serological Testing / SARS-CoV-2 / Antigens, Viral Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Limits: Humans Language: English Journal: Bioengineered Year: 2022 Document Type: Article Affiliation country: 21655979.2021.2014385