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Alteration of the gut microbiota following SARS-CoV-2 infection correlates with disease severity in hamsters.
Sencio, Valentin; Machelart, Arnaud; Robil, Cyril; Benech, Nicolas; Hoffmann, Eik; Galbert, Chloé; Deryuter, Lucie; Heumel, Séverine; Hantute-Ghesquier, Aline; Flourens, Anne; Brodin, Priscille; Infanti, Fabrice; Richard, Virgile; Dubuisson, Jean; Grangette, Corinne; Sulpice, Thierry; Wolowczuk, Isabelle; Pinet, Florence; Prévot, Vincent; Belouzard, Sandrine; Briand, François; Duterque-Coquillaud, Martine; Sokol, Harry; Trottein, François.
  • Sencio V; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.
  • Machelart A; Centre National de la Recherche Scientifique (CNRS), UMR 9017, F-59000 Lille, France.
  • Robil C; Institut National de la Santé et de la Recherche Médicale (Inserm) U1019, F-59000 Lille, France.
  • Benech N; Centre Hospitalier Universitaire de Lille, F-59000 Lille, France.
  • Hoffmann E; Institut Pasteur de Lille, F-59000 Lille, France.
  • Galbert C; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.
  • Deryuter L; Centre National de la Recherche Scientifique (CNRS), UMR 9017, F-59000 Lille, France.
  • Heumel S; Institut National de la Santé et de la Recherche Médicale (Inserm) U1019, F-59000 Lille, France.
  • Hantute-Ghesquier A; Centre Hospitalier Universitaire de Lille, F-59000 Lille, France.
  • Flourens A; Institut Pasteur de Lille, F-59000 Lille, France.
  • Brodin P; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.
  • Infanti F; Centre National de la Recherche Scientifique (CNRS), UMR 9017, F-59000 Lille, France.
  • Richard V; Institut National de la Santé et de la Recherche Médicale (Inserm) U1019, F-59000 Lille, France.
  • Dubuisson J; Centre Hospitalier Universitaire de Lille, F-59000 Lille, France.
  • Grangette C; Institut Pasteur de Lille, F-59000 Lille, France.
  • Sulpice T; Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Saint Antoine Hospital, Gastroenterology department, F-75012 Paris, France.
  • Wolowczuk I; Paris Center for Microbiome Medicine, Fédération Hospitalo-Universitaire, F-75012 Paris, France.
  • Pinet F; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 9017 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.
  • Prévot V; Centre National de la Recherche Scientifique (CNRS), UMR 9017, F-59000 Lille, France.
  • Belouzard S; Institut National de la Santé et de la Recherche Médicale (Inserm) U1019, F-59000 Lille, France.
  • Briand F; Centre Hospitalier Universitaire de Lille, F-59000 Lille, France.
  • Duterque-Coquillaud M; Institut Pasteur de Lille, F-59000 Lille, France.
  • Sokol H; Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Saint Antoine Hospital, Gastroenterology department, F-75012 Paris, France.
  • Trottein F; Paris Center for Microbiome Medicine, Fédération Hospitalo-Universitaire, F-75012 Paris, France.
Gut Microbes ; 14(1): 2018900, 2022.
Article in English | MEDLINE | ID: covidwho-1585291
ABSTRACT
Mounting evidence suggests that the gut-to-lung axis is critical during respiratory viral infections. We herein hypothesized that disruption of gut homeostasis during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may associate with early disease outcomes. To address this question, we took advantage of the Syrian hamster model. Our data confirmed that this model recapitulates some hallmark features of the human disease in the lungs. We further showed that SARS-CoV-2 infection associated with mild intestinal inflammation, relative alteration in intestinal barrier property and liver inflammation and altered lipid metabolism. These changes occurred concomitantly with an alteration of the gut microbiota composition over the course of infection, notably characterized by a higher relative abundance of deleterious bacterial taxa such as Enterobacteriaceae and Desulfovibrionaceae. Conversely, several members of the Ruminococcaceae and Lachnospiraceae families, including bacteria known to produce the fermentative products short-chain fatty acids (SCFAs), had a reduced relative proportion compared to non-infected controls. Accordingly, infection led to a transient decrease in systemic SCFA amounts. SCFA supplementation during infection had no effect on clinical and inflammatory parameters. Lastly, a strong correlation between some gut microbiota taxa and clinical and inflammation indices of SARS-CoV-2 infection severity was evidenced. Collectively, alteration of the gut microbiota correlates with disease severity in hamsters making this experimental model valuable for the design of interventional, gut microbiota-targeted, approaches for the control of COVID-19.Abbreviations SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; COVID-19, coronavirus disease 2019; SCFAs, short-chain fatty acids; dpi, day post-infection; RT-PCR, reverse transcription polymerase chain reaction; IL, interleukin. ACE2, angiotensin converting enzyme 2; TMPRSS2, transmembrane serine protease 2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mesocricetus / Disease Models, Animal / Gastrointestinal Microbiome / COVID-19 Type of study: Prognostic study Limits: Animals / Humans / Male Language: English Journal: Gut Microbes Year: 2022 Document Type: Article Affiliation country: 19490976.2021.2018900

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mesocricetus / Disease Models, Animal / Gastrointestinal Microbiome / COVID-19 Type of study: Prognostic study Limits: Animals / Humans / Male Language: English Journal: Gut Microbes Year: 2022 Document Type: Article Affiliation country: 19490976.2021.2018900