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Menthol-assisted homogenous liquid-liquid microextraction for HPLC/UV determination of favipiravir as an antiviral for COVID-19 in human plasma.
Abdallah, Inas A; Hammad, Sherin F; Bedair, Alaa; Mansour, Fotouh R.
  • Abdallah IA; Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City 32958, Egypt. Electronic address: Inas.Abdallah@fop.usc.edu.eg.
  • Hammad SF; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt.
  • Bedair A; Department of Analytical Chemistry, Faculty of Pharmacy, University of Sadat City, Sadat City 32958, Egypt.
  • Mansour FR; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt; Pharmaceutical Services Center, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt. Electronic address: fotouhrashed@pharm.tanta.edu.eg.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1189: 123087, 2022 Jan 15.
Article in English | MEDLINE | ID: covidwho-1587335
ABSTRACT
Favipiravir is a promising antiviral agent that has been recently approved for treatment of COVID-19 infection. In this study, a menthol-assisted homogenous liquid-liquid microextraction method has been developed for favipiravir determination in human plasma using HPLC/UV. The different factors that could affect the extraction efficiency were studied, including extractant type, extractant volume, menthol amount and vortex time. The optimum extraction efficiency was achieved using 300 µL of tetrahydrofuran, 30 mg of menthol and vortexing for 1 min before centrifuging the sample for 5 min at 3467g. Addition of menthol does not only induce phase separation, but also helps to form reverse micelles to facilitate extraction. The highly polar favipiravir molecules would be incorporated into the hydrophilic core of the formed reverse micelle to be extracted by the non-polar organic extractant. The method was validated according to the FDA bioanalytical method guidelines. The developed method was found linear in the concentration range of 0.1 to 100 µg/mL with a coefficient of determination of 0.9992. The method accuracy and precision were studied by calculating the recovery (%) and the relative standard deviation (%), respectively. The recovery (%) was in the range of 97.1-103.9%, while the RSD (%) values ranged between 2.03 and 8.15 %. The developed method was successfully applied in a bioequivalence study of Flupirava® 200 mg versus Avigan® 200 mg, after a single oral dose of favipiravir administered to healthy adult volunteers. The proposed method was simple, cheap, more eco-friendly and sufficiently sensitive for biomedical application.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Pyrazines / Liquid Phase Microextraction / Amides / COVID-19 Type of study: Evaluation study / Clinical Practice Guide Limits: Humans Language: English Journal: J Chromatogr B Analyt Technol Biomed Life Sci Journal subject: Biomedical Engineering Year: 2022 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Pyrazines / Liquid Phase Microextraction / Amides / COVID-19 Type of study: Evaluation study / Clinical Practice Guide Limits: Humans Language: English Journal: J Chromatogr B Analyt Technol Biomed Life Sci Journal subject: Biomedical Engineering Year: 2022 Document Type: Article