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Evaluation of serological anti-SARS-CoV-2 chemiluminescent immunoassays correlated to live virus neutralization test, for the detection of anti-RBD antibodies as a relevant alternative in COVID-19 large-scale neutralizing activity monitoring.
Cristiano, Antonio; Pieri, Massimo; Sarubbi, Serena; Pelagalli, Martina; Calugi, Graziella; Tomassetti, Flaminia; Bernardini, Sergio; Nuccetelli, Marzia.
  • Cristiano A; Department of Experimental Medicine, Tor Vergata University, Rome, Italy; Department of Biomedicine and Prevention, Tor Vergata University, Rome, Italy.
  • Pieri M; Department of Experimental Medicine, Tor Vergata University, Rome, Italy.
  • Sarubbi S; Department of Experimental Medicine, Tor Vergata University, Rome, Italy.
  • Pelagalli M; Department of Experimental Medicine, Tor Vergata University, Rome, Italy.
  • Calugi G; Lifebrain srl, Rome, Italy.
  • Tomassetti F; Lifebrain srl, Rome, Italy.
  • Bernardini S; Department of Experimental Medicine, Tor Vergata University, Rome, Italy; Tor Vergata University Hospital, Rome, Italy; IFCC Emerging Technologies Division, Milan, Italy.
  • Nuccetelli M; Department of Experimental Medicine, Tor Vergata University, Rome, Italy; Tor Vergata University Hospital, Rome, Italy. Electronic address: marzianuccetelli@yahoo.com.
Clin Immunol ; 234: 108918, 2022 01.
Article in English | MEDLINE | ID: covidwho-1588088
ABSTRACT
The Spike-Receptor Binding Domain (S-RBD) is considered the most antigenic protein in SARS-CoV-2 and probably the key player in SARS-CoV-2 immune response. Quantitative immunoassays may help establish an anti-RBD Abs threshold as an indication of protective immunity. Since different immunoassays are commercial, the standard reference method for the neutralizing activity is the live Virus Neutralization Test (VNT). In this study, anti-RBD IgG levels were detected with two chemiluminescent immunoassays in paucisymptomatic, symptomatic and vaccinated subjects, and their neutralizing activity was correlated to VNT titer, using SARS-CoV-2 original and British variant strains. Both immunoassays confirmed higher anti-RBD Abs levels in vaccinated subjects. Furthermore, despite different anti-RBD Abs median concentrations between the immunoassays, a strong positive correlation with VNT was observed. In conclusion, although the SARS-CoV-2 immune response heterogeneity, the use of immunoassays can help in large-scale monitoring of COVID-19 samples, becoming a valid alternative to VNT test for diagnostic routine laboratories.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoassay / Neutralization Tests / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines / Variants Limits: Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Clin Immunol Journal subject: Allergy and Immunology Year: 2022 Document Type: Article Affiliation country: J.clim.2021.108918

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoassay / Neutralization Tests / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Vaccines / Variants Limits: Adult / Aged / Animals / Female / Humans / Male / Middle aged / Young adult Language: English Journal: Clin Immunol Journal subject: Allergy and Immunology Year: 2022 Document Type: Article Affiliation country: J.clim.2021.108918