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Detection of SARS-CoV-2-RNA in post-mortem samples of human eyes.
Penkava, Josef; Muenchhoff, Maximilian; Badell, Irina; Osterman, Andreas; Delbridge, Claire; Niederbuchner, Florian; Soliman, Sarah; Rudelius, Martina; Graf, Alexander; Krebs, Stefan; Blum, Helmut; Ulbig, Michael; Baumann, Carmen; Zapp, Daniel; Maier, Mathias; Keppler, Oliver T; Lohmann, Chris P; Ledderose, Stephan.
  • Penkava J; Department of Ophthalmology, Technical University Munich, Munich, Germany. Josef.penkava@mri.tum.de.
  • Muenchhoff M; Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
  • Badell I; German Center for Infection Research, Partner Site Munich, Munich, Germany.
  • Osterman A; Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
  • Delbridge C; Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
  • Niederbuchner F; Department of Pathology and Neuropathology, TUM School of Medicine, Technical University Munich, Munich, Germany.
  • Soliman S; Department of Internal Medicine, Kliniken Südostbayern, Trostberg, Germany.
  • Rudelius M; Department of Pathology, Ludwig-Maximilian University Munich, Munich, Germany.
  • Graf A; Department of Pathology, Ludwig-Maximilian University Munich, Munich, Germany.
  • Krebs S; Laboratory for Functional Genome Analysis, Gene Center, Ludwig-Maximilians-University, Munich, Germany.
  • Blum H; Laboratory for Functional Genome Analysis, Gene Center, Ludwig-Maximilians-University, Munich, Germany.
  • Ulbig M; Laboratory for Functional Genome Analysis, Gene Center, Ludwig-Maximilians-University, Munich, Germany.
  • Baumann C; Department of Ophthalmology, Technical University Munich, Munich, Germany.
  • Zapp D; Department of Ophthalmology, Technical University Munich, Munich, Germany.
  • Maier M; Department of Ophthalmology, Technical University Munich, Munich, Germany.
  • Keppler OT; Department of Ophthalmology, Technical University Munich, Munich, Germany.
  • Lohmann CP; Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, LMU München, Munich, Germany.
  • Ledderose S; German Center for Infection Research, Partner Site Munich, Munich, Germany.
Graefes Arch Clin Exp Ophthalmol ; 260(5): 1789-1797, 2022 May.
Article in English | MEDLINE | ID: covidwho-1787813
ABSTRACT

PURPOSE:

To detect SARS-CoV-2 RNA in post-mortem human eyes. Ocular symptoms are common in patients with COVID-19. In some cases, they can occur before the onset of respiratory and other symptoms. Accordingly, SARS-CoV-2 RNA has been detected in conjunctival samples and tear film of patients suffering from COVID-19. However, the detection and clinical relevance of intravitreal SARS-CoV-2 RNA still remain unclear due to so far contradictory reports in the literature.

METHODS:

In our study 20 patients with confirmed diagnosis of COVID-19 were evaluated post-mortem to assess the conjunctival and intraocular presence of SARS-CoV-2 RNA using sterile pulmonary and conjunctival swabs as well as intravitreal biopsies (IVB) via needle puncture. SARS-CoV-2 PCR and whole genome sequencing from the samples of the deceased patients were performed. Medical history and comorbidities of all subjects were recorded and analyzed for correlations with viral data.

RESULTS:

SARS-CoV-2 RNA was detected in 10 conjunctival (50%) and 6 vitreal (30%) samples. SARS-CoV-2 whole genome sequencing showed the distribution of cases largely reflecting the frequency of circulating lineages in the Munich area at the time of examination with no preponderance of specific variants. Especially there was no association between the presence of SARS-CoV-2 RNA in IVBs and infection with the variant of concern (VOC) alpha. Viral load in bronchial samples correlated positively with load in conjunctiva but not the vitreous.

CONCLUSION:

SARS-CoV-2 RNA can be detected post mortem in conjunctival tissues and IVBs. This is relevant to the planning of ophthalmologic surgical procedures in COVID-19 patients, such as pars plana vitrectomy or corneal transplantation. Furthermore, not only during surgery but also in an outpatient setting it is important to emphasize the need for personal protection in order to avoid infection and spreading of SARS-CoV-2. Prospective studies are needed, especially to determine the clinical relevance of conjunctival and intravitreal SARS-CoV-2 detection concerning intraocular affection in active COVID-19 state and in post-COVID syndrome.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Risk factors Limits: Humans Language: English Journal: Graefes Arch Clin Exp Ophthalmol Year: 2022 Document Type: Article Affiliation country: S00417-021-05529-x

Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Risk factors Limits: Humans Language: English Journal: Graefes Arch Clin Exp Ophthalmol Year: 2022 Document Type: Article Affiliation country: S00417-021-05529-x